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Recombinant Drosophila melanogaster Profilin (chic)

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  • 中文名稱:
    Recombinant Drosophila melanogaster Profilin (chic)
  • 品名簡稱:
    Recombinant Drosophila melanogaster chic protein
  • 貨號:
    CSB-EP333112DLU
  • 說明書:
  • 規(guī)格:
    ¥1344
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
    chic
  • Uniprot No.:
  • 別名:
    Protein chickadee
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    20.6 kDa
  • 表達區(qū)域:
    1-126aa
  • 氨基酸序列
    MSWQDYVDNQLLASQCVTKACIAGHDGNIWAQSSGFEVTKEELSKLISGFDQQDGLTSNGVTLAGQRYIYLSGTDRVVRAKLGRSGVHCMKTTQAVIVSIYEDPVQPQQAASVVEKLGDYLITCGY
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    C-terminal 6xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. This profilin is required for intercellular cytoplasm transport during Drosophila oogenesis.
  • 基因功能參考文獻:
    1. Insulin/Insulin-like growth factor signaling specifically activates the expression of the actin regulator chickadee, the Drosophila homolog of Profilin, which is essential for promoting the formation of actin extensions and migration through the egg chamber.and migration through the egg chamber. PMID: 29980565
    2. Chic regulates interactions across the somatic permeability barrier around the germline that is essential for Drosophila spermatogenesis. PMID: 25503408
    3. chickadee, the Drosophila gene that encodes profilin, is required cell autonomously to maintain germline stem cells, possibly facilitating localization or maintenance of E-cadherin to the germline stem cell-hub cell interface. PMID: 24346697
    4. Data show that chic-deficient larvae fail to form a robust actin cable along the wound edge and also fail to form normal filopodial and lamellipodial extensions into the wound gap. PMID: 22976306
    5. D-SCAR and profilin were identified as important regulators of phagocytosis in Drosophila PMID: 12919849
    6. FMRP1 binds the mRNA of the Drosophila profilin and negatively regulates Profilin protein expression PMID: 15964283

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  • 亞細胞定位:
    Cytoplasm, cytoskeleton.
  • 蛋白家族:
    Profilin family
  • 組織特異性:
    Expressed in ovary and head.
  • 數據庫鏈接:

    KEGG: dme:Dmel_CG9553

    STRING: 7227.FBpp0089196

    UniGene: Dm.4775



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