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  4. Recombinant Drosophila melanogaster Profilin (chic)

Recombinant Drosophila melanogaster Profilin (chic)

  • 中文名稱：
    Recombinant Drosophila melanogaster Profilin(chic)
  • 貨號：
    CSB-YP333112DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Profilin(chic)
  • 貨號：
    CSB-EP333112DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Profilin(chic)
  • 貨號：
    CSB-BP333112DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Profilin(chic)
  • 貨號：
    CSB-MP333112DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    chic
  • Uniprot No.：
  • 別名：
    chic; chi; CG9553; Profilin; Protein chickadee
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-126
  • 氨基酸序列
    MSWQDYVDNQ LLASQCVTKA CIAGHDGNIW AQSSGFEVTK EELSKLISGF DQQDGLTSNG VTLAGQRYIY LSGTDRVVRA KLGRSGVHCM KTTQAVIVSI YEDPVQPQQA ASVVEKLGDY LITCGY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. This profilin is required for intercellular cytoplasm transport during Drosophila oogenesis.
  • 基因功能參考文獻：
    1. Insulin/Insulin-like growth factor signaling specifically activates the expression of the actin regulator chickadee, the Drosophila homolog of Profilin, which is essential for promoting the formation of actin extensions and migration through the egg chamber.and migration through the egg chamber. PMID: 29980565
    2. Chic regulates interactions across the somatic permeability barrier around the germline that is essential for Drosophila spermatogenesis. PMID: 25503408
    3. chickadee, the Drosophila gene that encodes profilin, is required cell autonomously to maintain germline stem cells, possibly facilitating localization or maintenance of E-cadherin to the germline stem cell-hub cell interface. PMID: 24346697
    4. Data show that chic-deficient larvae fail to form a robust actin cable along the wound edge and also fail to form normal filopodial and lamellipodial extensions into the wound gap. PMID: 22976306
    5. D-SCAR and profilin were identified as important regulators of phagocytosis in Drosophila PMID: 12919849
    6. FMRP1 binds the mRNA of the Drosophila profilin and negatively regulates Profilin protein expression PMID: 15964283

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Profilin family
  • 組織特異性：
    Expressed in ovary and head.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG9553

    STRING: 7227.FBpp0089196

    UniGene: Dm.4775



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