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  4. Recombinant Drosophila melanogaster Otefin (Ote), partial

Recombinant Drosophila melanogaster Otefin (Ote), partial

  • 中文名稱：
    黑腹果蠅Ote重組蛋白
  • 貨號：
    CSB-YP321785DLU1
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Ote重組蛋白
  • 貨號：
    CSB-EP321785DLU1
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Ote重組蛋白
  • 貨號：
    CSB-EP321785DLU1-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Ote重組蛋白
  • 貨號：
    CSB-BP321785DLU1
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Ote重組蛋白
  • 貨號：
    CSB-MP321785DLU1
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Ote
  • Uniprot No.：
  • 別名：
    Ote; CG5581; Otefin; LEM domain-containing protein Otefin
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Inner nuclear membrane protein. Involved in the attachment of membrane vesicles to chromatin during nuclear assembly, and is probably required for centrosome maturation and cell cycle progression during mitosis. Essential for differentiation of certain tissues and the maintenance of progenitor cell populations. Required for the differentiation and maintenance of male and female germline stem cells (GSCs), as well as the maintenance of somatic cells in the GSC niche. This role is likely to be independent of the BMP (Dpp) pathway that negatively regulates bam transcription during GSC differentiation. During development, plays essential and redundant functions with the other LEM domain proteins; bocks and MAN1. Also has a redundant but important role with bocks during larval development.
  • 基因功能參考文獻：
    1. Ote is required autonomously for survival of male and female stem cell populations, as well as non-autonomously for maintenance of two somatic niches. Ote loss in ovary causes a decrease in cap cell number and altered signal transduction. Ote mutant males become prematurely sterile as they age. PMID: 27174470
    2. production of Bocks, but not dMAN1, rescued ote-/- sterility, demonstrating that Ote and Bocks are functional homologs PMID: 24700158
    3. We show that the primary defect in ote(-/-) GSCs is a block of differentiation, which ultimately leads to germ cell death. PMID: 23806619
    4. Phosphorylation of Ote at T63 is critical for mitotic progression. PMID: 22751930
    5. Data show that otefin is essential for germline stem cell (GSC) maintenance, is required for the Dpp/BMP signaling pathway to silence bam transcription, and that Ote physically interacts with Medea/Smad4 at the bam silencer element to regulate GSC fate. PMID: 18410727

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  • 亞細胞定位：
    Nucleus inner membrane; Peripheral membrane protein; Nucleoplasmic side. Nucleus, nucleoplasm. Cytoplasm. Chromosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 組織特異性：
    Expressed in all cell types of the germarium and testis. Expressed in nurse cells, follicle cells and oocytes.
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG5581

    STRING: 7227.FBpp0085947

    UniGene: Dm.3658



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