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  4. Recombinant Drosophila melanogaster DNA-binding protein Ewg (ewg), partial

Recombinant Drosophila melanogaster DNA-binding protein Ewg (ewg), partial

  • 中文名稱：
    黑腹果蠅ewg重組蛋白
  • 貨號：
    CSB-YP634506DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅ewg重組蛋白
  • 貨號：
    CSB-EP634506DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅ewg重組蛋白
  • 貨號：
    CSB-EP634506DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅ewg重組蛋白
  • 貨號：
    CSB-BP634506DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅ewg重組蛋白
  • 貨號：
    CSB-MP634506DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ewg
  • Uniprot No.：
  • 別名：
    ewg; CG3114DNA-binding protein Ewg; Protein erect wing
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    May function as a positive regulator of transcription in developing and differentiated neurons, regulating common aspects of neuronal differentiation and maintenance. Requirement in the CNS may be higher than in the peripheral system. Vital for development of the indirect flight muscles.
  • 基因功能參考文獻：
    1. we show that Ewg repression of Cyclin E expression is required for the arrest of myoblast proliferation and initiate myoblast fusion and terminal differentiation PMID: 27039019
    2. in the adult intestine, Ebd acts independently of Ewg in physiological Wnt signaling, but cooperates with Ewg to induce the hyperactivation of Wnt target gene expression following Apc1 loss. These findings have relevance for human tumorigenesis, as Jerky (JRK/JH8), the human Ebd homolog, promotes Wnt pathway hyperactivation and is overexpressed in colorectal, breast, and ovarian cancers PMID: 28708826
    3. Knockdown of the Drosophila Nrf1 and Nrf2 ortholog cap 'n' collar isoform-C, but not Hsf1, blocks the protective effect of ASC-JM17 on mutant AR-induced eye degeneration in flies. Our observations indicate that activation of the Nrf1/Nrf2 pathway is a viable option for pharmacological intervention in SBMA and potentially other polyglutamine diseases. PMID: 26962150
    4. Mitochondrial fusion is regulated spatiotemporally by Ewg through Opa1-like during indirect flight muscles differentiation and growth. PMID: 24198395
    5. ewg regulates mutually exclusive R8 rhodopsin expression. PMID: 23850772
    6. The ELAV-binding site of D. virilis is fully functional in regulating alternative splicing of ewg intron 6 in D. melanogaster. PMID: 21705751
    7. Erect Wing facilitates context-dependent Wnt/Wingless signaling by recruiting the cell-specific Armadillo-TCF adaptor Earthbound to chromatin. PMID: 22028028
    8. Differential activity of EWG transcription factor isoforms identifies a subset of differentially regulated genes important for synaptic growth regulation PMID: 20854801
    9. embryonic lethal, abnormal vision CG4262-PA forms a dodecameric complex on 135 nucleotides in the last ewg intron PMID: 16107705

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  • 亞細胞定位：
    Nucleus. Note=Not in nucleolus.
  • 蛋白家族：
    NRF1/Ewg family
  • 組織特異性：
    Isoform A is highly expressed in possibly all embryonic neurons and is enriched in adult heads. Other isoforms show similar expression at a much lower level. Transient expression in migrating myoblasts.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG3114

    STRING: 7227.FBpp0300529

    UniGene: Dm.4971



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