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  4. Recombinant Drosophila melanogaster CLIP-associating protein (chb), partial

Recombinant Drosophila melanogaster CLIP-associating protein (chb), partial

  • 中文名稱：
    Recombinant Drosophila melanogaster CLIP-associating protein(chb) ,partial
  • 貨號：
    CSB-YP868249DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster CLIP-associating protein(chb) ,partial
  • 貨號：
    CSB-EP868249DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster CLIP-associating protein(chb) ,partial
  • 貨號：
    CSB-EP868249DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster CLIP-associating protein(chb) ,partial
  • 貨號：
    CSB-BP868249DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster CLIP-associating protein(chb) ,partial
  • 貨號：
    CSB-MP868249DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    chb
  • Uniprot No.：
  • 別名：
    chb; CLASP; Mast; MESR7; orbit; CG32435CLIP-associating protein; Misexpression suppressor of ras 7; Protein Multiple asters; Mast; Protein Orbit; Protein chromosome bows
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for several aspects of mitotic spindle formation including the formation of the overlapping central spindle microtubules and kinetochore attachment. Required for the incorporation of tubulin subunits at the plus ends of kinetochore microtubules during poleward microtubule flux. Acts antagonistically to Klp10A and Klp67A to maintain metaphase spindle length. Also required for guidance of CNS axons downstream of Abl. May function to identify a subset of microtubules that probe the peripheral growth cone domain, where guidance signals exert their influence on cytoskeletal organization. Also required during oogenesis for the organization of the polarized microtubule network inside the 16-cell cyst that ensures oocyte differentiation.
  • 基因功能參考文獻：
    1. Orbit plays an essential role as a connector between microtubules and the contractile ring in Drosophila male meiosis. PMID: 24850412
    2. Orbit is involved in the proper orientation of spindles towards fusomes during synchronous mitosis of spermatogonial cysts. PMID: 23520495
    3. MAST/Orbit has a role in microtubule-kinetochore attachment and is essential for chromosome alignment and maintenance of spindle bipolarity. PMID: 12034769
    4. Data show that the Orbit/Mast microtubule-associated protein is required at several stages in Drosophila oocyte cell differentiation. PMID: 12538517
    5. Orbit/Mast/CLASP localizes to interior rather than peripheral central spindle microtubules PMID: 15240569
    6. is an essential component for microtubule subunit incorporation into fluxing K-fibres PMID: 15592460
    7. results suggest that Mast plays an essential role in reducing the dynamic behaviour of microtubules by specifically promoting the pause state PMID: 17487886
    8. Dynein and mast/orbit/CLASP have antagonistic roles in regulating kinetochore-microtubule plus-end dynamics. PMID: 19549687

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cell projection, growth cone. Cleavage furrow. Note=Localizes to punctate cytoplasmic foci in interphase. Concentrates on spindle microtubules from prophase to late anaphase. Accumulates on the mid-part of the central spindle during telophase where it remains until cleavage. Subsequently appears at the periphery of the reforming karyomeres. Also localizes to the spindle remnant, cleavage furrows and the fusome of the 16-cell ovarian cyst. Localizes to the axonal growth cone within neurons.
  • 蛋白家族：
    CLASP family
  • 組織特異性：
    Expressed in testis and ovary.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG32435

    STRING: 7227.FBpp0088469

    UniGene: Dm.1413



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