Recombinant Dictyostelium discoideum Protein VASP homolog (vasp)
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中文名稱:盤基網柄菌vasp重組蛋白
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貨號:CSB-EP735989DKK-B
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說明書:
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規格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
產品詳情
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純度:>85% (SDS-PAGE)
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基因名:
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Uniprot No.:
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別名:vasp; DDB_G0289541Protein VASP homolog; DdVASP
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種屬:Dictyostelium discoideum (Slime mold)
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蛋白長度:full length protein
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表達區域:1-380
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氨基酸序列MSETAIFNAT GQVFTYSPQT RNWVPSSNVP ATLQMYFNSG ANTYRVIGRA GDDPNNFLIN FAVKSEVVYS RASEIFHQFT DQRTHFGINF TSKQDADTFG GGFENVLRSL KGGPQQPPPQ VPKPQPQQPP QPQQPPQRPP STVIAKPVAP QAPVAPPQAP AAAPQAPAPP AAPPAPPKPP GPPPPPPAPK PPAAGGGTGR NALLGSIENF SKGGLKKTVT VDKSAGVPTK TTPSANSAAS NAGSEPSSGG STPAPAPKSS GGGGGGDLMA EVMAKRAKMK AAASQPKEES SAPTPAPTPA PTPAPTPSST SFKPPQSFSK PATKTAAANK PPSPSLSAPL PSTVANEDLQ SLKEEILTEV RKEIQKAKDE ILEAIRASQH
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蛋白標簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式:Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點詳情
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功能:Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as lamellipodial and filopodial dynamics in migrating cells. Plays a crucial role in filopodia formation, cell-substratum adhesion, and proper chemotaxis. Nucleates and bundles actin filaments. When complexed with fotH in filopodial tips, may support formin-mediated filament elongation by bundling nascent actin filaments.
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基因功能參考文獻:
- Vasodilator-stimulated phosphoprotein (vasP) is required for filopodium formation in Dictyostelium discoideum. PMID: 16675552
- Involved in accelerating actin filament elongation in Dictyostelium discoideum filopodia PMID: 18923426
- Analysis of chemotaxis of cells expressing VASP mutants showed that VASP phosphorylation is required for the establishment of cell polarity under a cAMP gradient. PMID: 20191567
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亞細胞定位:Cytoplasm, cell cortex. Cell projection, filopodium. Cytoplasm, cytoskeleton. Note=Localizes to the leading edge of migrating cells and to the tips of filipodia. Translocates to the cell cortex in response to cAMP chemoattractant stimulation.
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蛋白家族:Ena/VASP family
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數據庫鏈接:
KEGG: ddi:DDB_G0289541
STRING: 44689.DDB0229340
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