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  4. Recombinant Dictyostelium discoideum Adenylyltransferase and sulfurtransferase MOCS3 (mocs3)

Recombinant Dictyostelium discoideum Adenylyltransferase and sulfurtransferase MOCS3 (mocs3)

  • 中文名稱：
    盤基網柄菌mocs3重組蛋白
  • 貨號：
    CSB-YP681896DKK
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    盤基網柄菌mocs3重組蛋白
  • 貨號：
    CSB-EP681896DKK
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    盤基網柄菌mocs3重組蛋白
  • 貨號：
    CSB-EP681896DKK-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    盤基網柄菌mocs3重組蛋白
  • 貨號：
    CSB-BP681896DKK
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    盤基網柄菌mocs3重組蛋白
  • 貨號：
    CSB-MP681896DKK
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mocs3
  • Uniprot No.：
  • 別名：
    mocs3; uba4; DDB_G0267980; Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin-like protein activator 4 homolog) [Includes: Molybdopterin-synthase adenylyltransferase; EC 2.7.7.80; Adenylyltransferase MOCS3; Sulfur carrier protein MOCS2A adenylyltransferase); Molybdopterin-synthase sulfurtransferase; EC 2.8.1.11; Sulfur carrier protein MOCS2A sulfurtransferase; Sulfurtransferase MOCS3)]
  • 種屬：
    Dictyostelium discoideum (Slime mold)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-425
  • 氨基酸序列
    MEKYIKEHTL KPNEIERYGR QLITPDIGVS GQMSLCNSSV LIIGAGGLGC PVALYLSSAG IGTLGLVDYD TVEISNLHRQ IGHRESSKGI SKAVSLSKTI SELNSLIKVN TYETTFTSET AMEIIKNYDI VVDASDNVAT RYLVNDACVL TGKPLVSGSA LKWEGQITCY NYNNGPCYRC IFPTPPPVET VTKCSDGGVL GPIVGVIGSL QALEVIKILT NNKEGVLSGR LLIYDGMSAV FRTVRIRGKQ SGCNVCGDKP TVTQLIDYTQ FCQSNYSESA GKVDDRVDKT LIITVEQFNQ SIKNNNNNHL LIDVRPKIQF DICSLPNSNN IPIEEIDKKE SIEIIEKLIK EKVENNNNNN NNNENRDASD ELSVYLVCRR GNKSQDAVKI LDEKLKEFRD KFKLLHIKDG LLGWNESIDD SFPIY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and mocs2a. Its N-terminus first activates urm1 and mocs2a as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and mocs2a to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and mocs2a. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions.
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    HesA/MoeB/ThiF family, UBA4 subfamily
  • 數據庫鏈接：


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