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  4. Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7 (daf-7)

Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7 (daf-7)

  • 中文名稱：
    Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7(daf-7)
  • 貨號：
    CSB-YP311270CXY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7(daf-7)
  • 貨號：
    CSB-EP311270CXY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7(daf-7)
  • 貨號：
    CSB-BP311270CXY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7(daf-7)
  • 貨號：
    CSB-MP311270CXY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    daf-7
  • Uniprot No.：
  • 別名：
    daf-7; B0412.2; Dauer larva development regulatory growth factor daf-7; Abnormal dauer formation protein 7
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    235-350
  • 氨基酸序列
    SHAKPV CNAEAQSKGC CLYDLEIEFE KIGWDWIVAP PRYNAYMCRG DCHYNAHHFN LAETGHSKIM RAAHKVSNPE IGYCCHPTEY DYIKLIYVNR DGRVSIANVN GMIAKKCGCS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Under harsh environmental conditions, larvae enter a developmentally arrested state known as dauer; TGF-beta-like daf-7 acts to inhibit dauer larva formation and promote growth. May be a ligand to cell surface receptor daf-4. May act as a negative regulator of dauer larva development by transducing chemosensory information from ASI neurons. Involved in sensitivity to CO2 levels. In AWC neurons, acts to promote expression of srsx-3, a member of the GPCR family.
  • 基因功能參考文獻：
    1. DAF-7 expression decreases as animals age during adulthood. Age-dependent diminished expression contributes to the reduced sensitivity of aging animals to the effects of dietary restriction. PMID: 28107363
    2. Using functional, fluorescently-tagged protein, we find that, in animals with mutant DAF-28/IGF, the wild-type DAF-7/TGF-beta is mislocalized to and accumulates in the proximal axon of the ASI neuron. Activation of two different branches of the unfolded protein response can modulate both the developmental phenotype and DAF-7 mislocalization in DAF-28(R37C) animals, but appear to act through divergent mechanisms PMID: 27926939
    3. GLR-1 protein and glr-1 transcription are increased in neurons of DAF-7 mutants. PMID: 26054666
    4. daf-7 has a role in larval development and specifically affects molting and excretory canal development PMID: 23526825
    5. We show that aggregation and bordering are genetically complex, with multiple contributing quantitative trait loci (QTLs), and refine one QTL to identify a new gene affecting the daf-7 pathway, the GABA receptor EXP-1. PMID: 23284308

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 組織特異性：
    Expression in the chemosensory neurons.
  • 數據庫鏈接：

    KEGG: cel:CELE_B0412.2

    STRING: 6239.B0412.2

    UniGene: Cel.19630



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