在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Clostridium botulinum C phage Botulinum neurotoxin type C, partial

In Stock
  • 中文名稱:
    Recombinant Clostridium botulinum C phage Botulinum neurotoxin type C,partial
  • 品名簡稱:
    Recombinant Clostridium botulinum C phage Botulinum neurotoxin type C protein, partial
  • 貨號:
    CSB-EP325596CLQ
  • 說明書:
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP325596CLQ could indicate that this peptide derived from E.coli-expressed Clostridium botulinum N/A.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP325596CLQ could indicate that this peptide derived from E.coli-expressed Clostridium botulinum N/A.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    N/A
  • Uniprot No.:
  • 別名:
    Botulinum neurotoxin type C; BoNT/C; Bontoxilysin-C1; BoNT/C1; Botulinum neurotoxin type C1) [Cleaved into: Botulinum neurotoxin C light chain; LC; EC 3.4.24.69); Botulinum neurotoxin C heavy chain; HC)]
  • 種屬:
    Clostridium botulinum C phage (Clostridium botulinum C bacteriophage)
  • 蛋白長度:
    Partial
  • 來源:
    E.coli
  • 分子量:
    64.9kDa
  • 表達區域:
    2-428aa
  • 氨基酸序列
    PITINNFNYSDPVDNKNILYLDTHLNTLANEPEKAFRITGNIWVIPDRFSRNSNPNLNKPPRVTSPKSGYYDPNYLSTDSDKDPFLKEIIKLFKRINSREIGEELIYRLSTDIPFPGNNNTPINTFDFDVDFNSVDVKTRQGNNWVKTGSINPSVIITGPRENIIDPETSTFKLTNNTFAAQEGFGALSIISISPRFMLTYSNATNDVGEGRFSKSEFCMDPILILMHELNHAMHNLYGIAIPNDQTISSVTSNIFYSQYNVKLEYAEIYAFGGPTIDLIPKSARKYFEEKALDYYRSIAKRLNSITTANPSSFNKYIGEYKQKLIRKYRFVVESSGEVTVNRNKFVELYNELTQIFTEFNYAKIYNVQNRKIYLSNVYTPVTANILDDNVYDIQNGFNIPKSNLNVLFMGQNLSRNPALRKVNPEN
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-SUMO-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Is unique among characterized BoNTs in having 2 substrates, syntaxin (STX) and SNAP25. Precursor of botulinum neurotoxin C which unlike most BoNTs seems not to have a proteinaceous coreceptor, and instead recognizes 2 different complex polysialylated gangliosides found on neural tissue probably found in synaptic vesicles. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the heavy chain (HC) forms pores that allows the light chain (LC) to translocate into the cytosol. Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release. In vitro the whole toxin only has protease activity after reduction. Electrical stimulation increases uptake of toxin, presumably by transiently exposing a receptor usually found in eukaryotic target synaptic vesicles. Forms ion-conducting channels at around pH 6.1. Requires complex eukaryotic host polysialogangliosides for full neurotoxicity. Synaptic vesicle glycoproteins (SV2) do not seem to act as its receptor.; Has proteolytic activity. After translocation into the eukaryotic host cytosol, inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves syntaxin-1A/STX1A and syntaxin-1B/STX1B. Cleaves the '253-Arg-|-Ala-254' bond of STX1 and the '252-Arg-|-Ala-253' bond of STX2; also acts on syntaxin 3 (STX3) but not 4 (STX4). Cleaves the '198-Arg-|-Ala-199' bond of SNAP25. Recognizes the '93-Asn--Met-202' region of SNAP25.; Responsible for host epithelial cell transcytosis, host nerve cell targeting and translocation of light chain (LC) into eukaryotic host cytosol. Composed of 3 subdomains; the translocation domain (TD), and N-terminus and C-terminus of the receptor-binding domain (RBD). The RBD is responsible for the adherence of the toxin to the eukaryotic target cell surface. It simultaneously recognizes 2 polysialated gangliosides coreceptors in close proximity on host synaptic vesicles. The N-terminus of the TD wraps an extended belt around the perimeter of the LC, protecting Zn(2+) in the active site; it may also prevent premature LC dissociation from the translocation channel and protect toxin prior to translocation. The TD inserts into synaptic vesicle membrane to allow translocation into the host cytosol. The C-terminal half of the HC (residues 864-1291) binds neurons in a dose-dependent manner. The C-terminal half of the HC (residues 863-1291) binds eukaryotic host gangliosides in the order GD1b > GT1b > GD1a > GM1a. Has 2 ganglioside binding sites; Sia-1 prefers a sia7 sialic acid and sugars within the ganglioside (GD1b > GT1b), whereas GBP2 recognizes a sia5 sialic acid (GT1b and GD1a). Both sites are required for HC to enter neurons, acting via different gangliosides. This suggests that 2 gangliosides serve as toxin receptors. Synaptic activity (depolarization with K(+)) increases uptake by neurons. Treatment of synaptosomes with proteinase K does not reduce HC binding, suggesting there is no protein receptor or it is protected from extracellular proteases. Decreases uptake and toxicity of whole BoNT/A, but also interferes with uptake of BoNT/E and BoNT/F. HC also binds phosphoinositides, which might play a role in membrane-binding.
  • 亞細胞定位:
    [Botulinum neurotoxin type C]: Secreted.; [Botulinum neurotoxin C light chain]: Secreted.; [Botulinum neurotoxin C heavy chain]: Secreted.
  • 蛋白家族:
    Peptidase M27 family


主站蜘蛛池模板: 国产区精品福利在线社区| 麻豆成人久久精品二区三区免费| 色777狠狠狠综合| 色avav色av爱avav亚洲色拍| 亚洲伊人久久精品酒店| 狠狠色噜噜狠狠狠狠777米奇小说 又黄又爽的60分钟视频 | 越南女子杂交内射bbwxz| √天堂中文官网8在线| 国产精品白丝喷水在线观看 | 亚洲日韩av在线观看| 精品三级av无码一区| 欧美综合自拍亚洲图久青草| 亚洲人成小说网站色在线观看| 亚洲国产另类久久久精品| 无码人妻av一区二区三区蜜臀| 人妻无码一区二区三区av| 国产杨幂丝袜av在线播放| 强制高潮18xxxxhd日韩| 成人试看120秒体验区| 国产乱人伦偷精品视频| 曰韩欧美群交p片内射| 国产一卡2卡3卡4卡精品| 影音先锋中文字幕人妻| 国产肥熟女视频一区二区三区 | 少妇极品熟妇人妻无码| 欧美肥熟妇xxxxx| 久久天天躁夜夜躁狠狠躁2014| 大伊香蕉精品视频在线直播| 中文成人无字幕乱码精品区| 大战熟女丰满人妻av| 亚洲熟妇av午夜无码不卡| 国产精品免费视频色拍拍| 秋霞影院午夜伦a片欧美| 成人午夜国产内射主播| 天堂在线最新版资源www| 日本一区午夜艳熟免费| 5566先锋影音夜色资源站在线观看| 国产无套内射又大又猛又粗又爽| 丰满人妻被公侵犯完整版| 亚洲线精品一区二区三区| 亚洲精品成人无限看|