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  4. Recombinant Chlamydomonas reinhardtii Ribulose bisphosphate carboxylase large chain (rbcL)

Recombinant Chlamydomonas reinhardtii Ribulose bisphosphate carboxylase large chain (rbcL)

  • 中文名稱：
    Recombinant Chlamydomonas reinhardtii Ribulose bisphosphate carboxylase large chain(rbcL)
  • 貨號：
    CSB-YP365481DSJ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Chlamydomonas reinhardtii Ribulose bisphosphate carboxylase large chain(rbcL)
  • 貨號：
    CSB-EP365481DSJ
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Chlamydomonas reinhardtii Ribulose bisphosphate carboxylase large chain(rbcL)
  • 貨號：
    CSB-EP365481DSJ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Chlamydomonas reinhardtii Ribulose bisphosphate carboxylase large chain(rbcL)
  • 貨號：
    CSB-BP365481DSJ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Chlamydomonas reinhardtii Ribulose bisphosphate carboxylase large chain(rbcL)
  • 貨號：
    CSB-MP365481DSJ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rbcL
  • Uniprot No.：
  • 別名：
    rbcL; Ribulose bisphosphate carboxylase large chain; RuBisCO large subunit; EC 4.1.1.39
  • 種屬：
    Chlamydomonas reinhardtii (Chlamydomonas smithii)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    3-475
  • 氨基酸序列
    PQTETKAG AGFKAGVKDY RLTYYTPDYV VRDTDILAAF RMTPQLGVPP EECGAAVAAE SSTGTWTTVW TDGLTSLDRY KGRCYDIEPV PGEDNQYIAY VAYPIDLFEE GSVTNMFTSI VGNVFGFKAL RALRLEDLRI PPAYVKTFVG PPHGIQVERD KLNKYGRGLL GCTIKPKLGL SAKNYGRAVY ECLRGGLDFT KDDENVNSQP FMRWRDRFLF VAEAIYKAQA ETGEVKGHYL NATAGTCEEM MKRAVCAKEL GVPIIMHDYL TGGFTANTSL AIYCRDNGLL LHIHRAMHAV IDRQRNHGIH FRVLAKALRM SGGDHLHSGT VVGKLEGERE VTLGFVDLMR DDYVEKDRSR GIYFTQDWCS MPGVMPVASG GIHVWHMPAL VEIFGDDACL QFGGGTLGHP WGNAPGAAAN RVALEACTQA RNEGRDLARE GGDVIRSACK WSPELAAACE VWKEIKFEFD TIDKL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
  • 基因功能參考文獻：
    1. A novel MS based approach enabling the exact stoichiometric analysis and absolute quantification of protein complexes is presented in this article. The application of this method revealed new insights in RuBisCO subunit dynamics. PMID: 25301535
    2. In the absence of Rubisco and PGR5, a sustained electron flow is maintained with molecular oxygen instead of carbon dioxide serving as the terminal electron acceptor. PMID: 24623849
    3. A variety of subtle interactions must be responsible for catalytic efficiency and CO2/O2 specificity of rbcL. PMID: 17824672
    4. Results indicated that the two inverted repeats function as redundant mRNA stability determinants in the 3' UTR of rbcL. PMID: 17996019
    5. C172S Rubisco showed a critical denaturation temperature approx PMID: 18072944
    6. The quadruple-mutant enzyme has a 10% increase in CO2/O2 specificity and a lower carboxylation catalytic efficiency. PMID: 18664299

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  • 亞細胞定位：
    Plastid, chloroplast.
  • 蛋白家族：
    RuBisCO large chain family, Type I subfamily
  • 數據庫鏈接：


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