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  4. Recombinant Bovine Zinc finger RAD18 domain-containing protein C1orf124 homolog

Recombinant Bovine Zinc finger RAD18 domain-containing protein C1orf124 homolog

  • 中文名稱：
    牛SPRTN重組蛋白
  • 貨號：
    CSB-YP399770BO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛SPRTN重組蛋白
  • 貨號：
    CSB-EP399770BO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛SPRTN重組蛋白
  • 貨號：
    CSB-EP399770BO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛SPRTN重組蛋白
  • 貨號：
    CSB-BP399770BO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛SPRTN重組蛋白
  • 貨號：
    CSB-MP399770BO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SPRTN
  • Uniprot No.：
  • 別名：
    SPRTN; SprT-like domain-containing protein Spartan; Protein with SprT-like domain at the N terminus; Spartan
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-487
  • 氨基酸序列
    MDEDLVLALQ LQEEWNLQVS EREPAQEPLS LVDASWELVD PTPDLQGLFV LFNDRFFWGQ LEAVEVKWSV RMTLCAGICS YEGRGGMCSI RLSEPLLKLR PRKDLVETLL HEMIHAYLFV TNNDKDREGH GPEFCKHMHR INRLTGANIT VYHTFHDEVD EYRRHWWRCN GPCQNSKPYY GYVKRATNRA PSAHDYWWAE HQKTCGGTYI KIKEPENYSK KGKGKTKLRK QPVSEAENKD KPNRGEKQLL IPFTGKGYVL GETSNFSSGK CITSHAINES QEPLSQDHSA NALRPHSKTE VKFEQNGPSK KTSVASPVLS TSHQNVLSNY FSKVSVASSK AFRSVSGSPV KSLTVGDSTT KSVSAGSQRR VTSSRTSLRN SLKAMESTYV TVPQDAGGPE GKLPSKRPRI EDKTFFDLFF IKKEQAQSGG GDVTSSSHPP AAAQSPSGAS GQSRVVHCPV CQDEVSETQI NEHLDWCLER DSTQVKS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity. DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde. Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis. Acts as a pleiotropic protease for DNA-binding proteins cross-linked with DNA, such as TOP1, TOP2A, histones H3 and H4. Mediates degradation of DPCs that are not ubiquitinated, while it is not able to degrade ubiquitinated DPCs. SPRTN activation requires polymerase collision with DPCs followed by helicase bypass of DPCs. Involved in recruitment of VCP/p97 to sites of DNA damage. Also acts as an activator of CHEK1 during normal DNA replication by mediating proteolytic cleavage of CHEK1, thereby promoting CHEK1 removal from chromatin and subsequent activation. Does not activate CHEK1 in response to DNA damage. May also act as a 'reader' of ubiquitinated PCNA: recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis.
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Spartan family
  • 數據庫鏈接：


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