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  4. Recombinant Bovine E3 ubiquitin-protein ligase RNF34 (RNF34)

Recombinant Bovine E3 ubiquitin-protein ligase RNF34 (RNF34)

  • 中文名稱：
    牛RNF34重組蛋白
  • 貨號：
    CSB-YP707812BO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛RNF34重組蛋白
  • 貨號：
    CSB-EP707812BO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛RNF34重組蛋白
  • 貨號：
    CSB-EP707812BO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛RNF34重組蛋白
  • 貨號：
    CSB-BP707812BO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛RNF34重組蛋白
  • 貨號：
    CSB-MP707812BO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RNF34
  • Uniprot No.：
  • 別名：
    RNF34; E3 ubiquitin-protein ligase RNF34; EC 2.3.2.27; RING finger protein 34; RING-type E3 ubiquitin transferase RNF34
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-375
  • 氨基酸序列
    MKAGATSMWA SCCGLLNEVM GTGAVRGQQS GFAGGTGPFR FTPNSDFSAY PPASAEGPNI VCKACGLSFS VFRKKHVCCD CKKDFCSVCS VLQENLRRCS TCHLLQETAF QRPQLMRLKV KDLRQYLILR NIPIDTCREK EDLVDLVLCH HRLGSEDDLD TSSLNSSRSQ TSSFFTHSFF SNYTAPSATA SSFQGELMGG DRTLGSGALA QEPSEIASAN TEDDEDDDDD DDDDDDDDEE NLEDRTPGLT KKRVRASLSD LSSLEDVEGM SVRQLKEILA RNFVNYSGCC EKWELVEKVN RLYKENEENQ KSYGERLQLQ DEEDDSLCRI CMDAVIDCVL LECGHMVTCT KCGKRMSECP ICRQYVVRAV HVFKS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells. Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation.
  • 亞細胞定位：
    Cell membrane; Peripheral membrane protein. Endomembrane system; Peripheral membrane protein. Nucleus. Nucleus speckle. Cytoplasm, cytosol.
  • 數據庫鏈接：


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