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Recombinant Bombyx mori Prothoracicotropic hormone

  • 中文名稱:
    Recombinant Bombyx mori Prothoracicotropic hormone
  • 貨號:
    CSB-EP324609BTT
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    N/A
  • Uniprot No.:
  • 別名:
    ; Prothoracicotropic hormone; PTTH) [Cleaved into: P2K; P6K; Prothoracicotropic hormone]
  • 種屬:
    Bombyx mori (Silk moth)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    16.7kDa
  • 表達區域:
    116-224aa
  • 氨基酸序列
    GNIQVENQAIPDPPCTCKYKKEIEDLGENSVPRFIETRNCNKTQQPTCRPPYICKESLYSITILKRRETKSQESLEIPNELKYRWVAESHPVSVACLCTRDYQLRYNNN
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    PTTH is a brain secretory polypeptide of insects which stimulates the prothoracic glands to produce and release ecdysone, the steroid essential to insect development.; Peptides P2K and P6K are presumed to be cleaved post-translationally and may play some unknown physiologically or developmentally important functions.
  • 基因功能參考文獻:
    1. HR38 is a prothoracicotropic hormone(PTTH)-stimulated immediate early gene that is, at least in part, induced through Ca(2+)/ERK and PI3K signaling. The present study proposes a potential cross talk mechanism between PTTH and ecdysone signaling to regulate insect development and lays a foundation for a better understanding of the mechanisms of PTTH's actions. PMID: 27090809
    2. There are interconnections among Ca(2+) signaling, adenosine 5'-monophosphate-activated protein kinase, and 4E-BP phosphorylation in in prothoracicotropic hormone (PTTH-activated prothoracic glands of Bombyx mori. PMID: 27751888
    3. Study reports the physical basis of neurohormone PTTH sensing by the receptor tyrosine kinase Torso. Hormone binding by Torso is asymmetric and anticooperative, allowing receptor levels to determine the nature of signal transduction. PMID: 26698662
    4. To examine the expression of Rab proteins and neuropeptide hormones in the brain of the silkworm, Bombyx mori.The Rab1 co-localized with prothoracicotropic hormone. PMID: 22922733
    5. PTTH/PI3K/TOR/4E-BP (S6K) signaling is involved in PTTH-stimulated ecdysteroidogenesis by prothoracic glands. PMID: 22227406
    6. The authors showed that Bombyx mori prothoracicotropic hormone (PTTH)-stimulated ecdysteroidogenesis was partially blocked by LY294002 and wortmannin, indicating that phosphoinositide 3-kinase (PI3K) is involved in PTTH-stimulated ecdysteroidogenesis. PMID: 21199670
    7. Study showed that prothoracicotropic hormone (PTTH) stimulates a rapid increase in tyrosine phosphorylation of at least 2 proteins in prothoracic glands: extracellular signal-regulated kinase and a 120-kDa protein. PMID: 20568296
    8. the TATA box is involved in regulation of PTTH gene expression in B. mori PMID: 20563654
    9. PTTH-stimulated ERK phosphorylation is only partially Ca(2+)- and calmodulin-dependent, and HNMPA-(AM)(3)-sensitive receptor tyrosine kinase is involved in activation of ERK phosphorylation by PTTH. PMID: 19800889

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  • 組織特異性:
    PTTH is synthesized by two dorsolateral neurosecretory cells of the Bombyx brain.
  • 數據庫鏈接:

    KEGG: bmor:692767

    UniGene: Bmo.125



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