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  4. Recombinant Bombyx mori Prothoracicotropic hormone

Recombinant Bombyx mori Prothoracicotropic hormone

  • 中文名稱：
    Recombinant Bombyx mori Prothoracicotropic hormone
  • 貨號：
    CSB-YP324609BTT
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Bombyx mori Prothoracicotropic hormone
  • 貨號：
    CSB-EP324609BTT-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Bombyx mori Prothoracicotropic hormone
  • 貨號：
    CSB-BP324609BTT
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Bombyx mori Prothoracicotropic hormone
  • 貨號：
    CSB-MP324609BTT
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 別名：
    ; Prothoracicotropic hormone; PTTH) [Cleaved into: P2K; P6K; Prothoracicotropic hormone]
  • 種屬：
    Bombyx mori (Silk moth)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    116-224
  • 氨基酸序列
    GNIQV ENQAIPDPPC TCKYKKEIED LGENSVPRFI ETRNCNKTQQ PTCRPPYICK ESLYSITILK RRETKSQESL EIPNELKYRW VAESHPVSVA CLCTRDYQLR YNNN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    PTTH is a brain secretory polypeptide of insects which stimulates the prothoracic glands to produce and release ecdysone, the steroid essential to insect development.; Peptides P2K and P6K are presumed to be cleaved post-translationally and may play some unknown physiologically or developmentally important functions.
  • 基因功能參考文獻：
    1. HR38 is a prothoracicotropic hormone(PTTH)-stimulated immediate early gene that is, at least in part, induced through Ca(2+)/ERK and PI3K signaling. The present study proposes a potential cross talk mechanism between PTTH and ecdysone signaling to regulate insect development and lays a foundation for a better understanding of the mechanisms of PTTH's actions. PMID: 27090809
    2. There are interconnections among Ca(2+) signaling, adenosine 5'-monophosphate-activated protein kinase, and 4E-BP phosphorylation in in prothoracicotropic hormone (PTTH-activated prothoracic glands of Bombyx mori. PMID: 27751888
    3. Study reports the physical basis of neurohormone PTTH sensing by the receptor tyrosine kinase Torso. Hormone binding by Torso is asymmetric and anticooperative, allowing receptor levels to determine the nature of signal transduction. PMID: 26698662
    4. To examine the expression of Rab proteins and neuropeptide hormones in the brain of the silkworm, Bombyx mori.The Rab1 co-localized with prothoracicotropic hormone. PMID: 22922733
    5. PTTH/PI3K/TOR/4E-BP (S6K) signaling is involved in PTTH-stimulated ecdysteroidogenesis by prothoracic glands. PMID: 22227406
    6. The authors showed that Bombyx mori prothoracicotropic hormone (PTTH)-stimulated ecdysteroidogenesis was partially blocked by LY294002 and wortmannin, indicating that phosphoinositide 3-kinase (PI3K) is involved in PTTH-stimulated ecdysteroidogenesis. PMID: 21199670
    7. Study showed that prothoracicotropic hormone (PTTH) stimulates a rapid increase in tyrosine phosphorylation of at least 2 proteins in prothoracic glands: extracellular signal-regulated kinase and a 120-kDa protein. PMID: 20568296
    8. the TATA box is involved in regulation of PTTH gene expression in B. mori PMID: 20563654
    9. PTTH-stimulated ERK phosphorylation is only partially Ca(2+)- and calmodulin-dependent, and HNMPA-(AM)(3)-sensitive receptor tyrosine kinase is involved in activation of ERK phosphorylation by PTTH. PMID: 19800889

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  • 組織特異性：
    PTTH is synthesized by two dorsolateral neurosecretory cells of the Bombyx brain.
  • 數據庫鏈接：

    KEGG: bmor:692767

    UniGene: Bmo.125



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