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  4. Recombinant Bacillus subtilis Stage 0 sporulation protein J (spo0J)

Recombinant Bacillus subtilis Stage 0 sporulation protein J (spo0J)

  • 中文名稱：
    枯草芽孢桿菌spo0J重組蛋白
  • 貨號：
    CSB-YP328624BRJ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌spo0J重組蛋白
  • 貨號：
    CSB-EP328624BRJ
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌spo0J重組蛋白
  • 貨號：
    CSB-EP328624BRJ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌spo0J重組蛋白
  • 貨號：
    CSB-BP328624BRJ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌spo0J重組蛋白
  • 貨號：
    CSB-MP328624BRJ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    spo0J
  • Uniprot No.：
  • 別名：
    spo0J; BSU40960; Stage 0 sporulation protein J
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-282
  • 氨基酸序列
    MAKGLGKGIN ALFNQVDLSE ETVEEIKIAD LRPNPYQPRK HFDDEALAEL KESVLQHGIL QPLIVRKSLK GYDIVAGERR FRAAKLAGLD TVPAIVRELS EALMREIALL ENLQREDLSP LEEAQAYDSL LKHLDLTQEQ LAKRLGKSRP HIANHLRLLT LPENIQQLIA EGTLSMGHGR TLLGLKNKNK LEPLVQKVIA EQLNVRQLEQ LIQQLNQNVP RETKKKEPVK DAVLKERESY LQNYFGTTVN IKRQKKKGKI EIEFFSNEDL DRILELLSER ES
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for the initiation of sporulation and for normal chromosome segregation. Antagonizes sporulation inhibition by Soj. It probably interacts with a specific DNA site and other proteins involved in partitioning and cell division, and antagonizes Soj in response to cell cycle events related to chromosome partitioning.
  • 基因功能參考文獻：
    1. A network of cis and trans interactions is required for ParB (Spo0J) spreading. PMID: 28407103
    2. Targeting of Smc/ScpAB to ParB/parS loading sites is strictly dependent on engagement of Smc head domains and relies on an open organization of the Smc coiled coils. PMID: 26904953
    3. Non-specific binding of DNA by ParB leads to condensation. PMID: 25572315
    4. Findings indicate that spreading is important for in vivo function of Spo0J. PMID: 17462018
    5. Study demonstrates that structural maintenance of chromosomes is highly enriched in the region around the origin of replication, specifically near parS sites to which Spo0J binds and at highly transcribed genes. PMID: 19450516
    6. In Bacillus subtilis, parS sites are bound by the partitioning protein Spo0J (ParB), and we show here that Spo0J recruits the structural maintenance of chromosomes complex to the origin. PMID: 19450517

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  • 蛋白家族：
    ParB family
  • 數據庫鏈接：


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