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  4. Recombinant Arabidopsis thaliana Protein phosphatase 2C 56 (ABI1)

Recombinant Arabidopsis thaliana Protein phosphatase 2C 56 (ABI1)

  • 中文名稱：
    Recombinant Arabidopsis thaliana Protein phosphatase 2C 56(ABI1)
  • 貨號：
    CSB-YP344213DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Protein phosphatase 2C 56(ABI1)
  • 貨號：
    CSB-EP344213DOA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Protein phosphatase 2C 56(ABI1)
  • 貨號：
    CSB-EP344213DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Protein phosphatase 2C 56(ABI1)
  • 貨號：
    CSB-BP344213DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Protein phosphatase 2C 56(ABI1)
  • 貨號：
    CSB-MP344213DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ABI1
  • Uniprot No.：
  • 別名：
    ABI1; At4g26080; F20B18.190; Protein phosphatase 2C 56; AtPP2C56; EC 3.1.3.16; Protein ABSCISIC ACID-INSENSITIVE 1; Protein phosphatase 2C ABI1; PP2C ABI1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-434
  • 氨基酸序列
    MEEVSPAIAG PFRPFSETQM DFTGIRLGKG YCNNQYSNQD SENGDLMVSL PETSSCSVSG SHGSESRKVL ISRINSPNLN MKESAAADIV VVDISAGDEI NGSDITSEKK MISRTESRSL FEFKSVPLYG FTSICGRRPE MEDAVSTIPR FLQSSSGSML DGRFDPQSAA HFFGVYDGHG GSQVANYCRE RMHLALAEEI AKEKPMLCDG DTWLEKWKKA LFNSFLRVDS EIESVAPETV GSTSVVAVVF PSHIFVANCG DSRAVLCRGK TALPLSVDHK PDREDEAARI EAAGGKVIQW NGARVFGVLA MSRSIGDRYL KPSIIPDPEV TAVKRVKEDD CLILASDGVW DVMTDEEACE MARKRILLWH KKNAVAGDAS LLADERRKEG KDPAAMSAAE YLSKLAIQRG SKDNISVVVV DLKPRRKLKS KPLN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as the actin reorganization in guard cells in response to ABA. Involved in the resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. Controls negatively fibrillin expression that is involved in mediating ABA-induced photoprotection. May be involved in ABA content regulation. Plays a role in the Pro accumulation in response to reduced water availability (low water potential). Required for the ABA negative regulation of the ethylene-induced hyponastic growth. Involved in acquired thermotolerance of root growth and seedling survival. Activates/represses SRK2E/OST1 in response to ABA-dependent stimuli, especially in stomata closure regulation involving SLAC1. Represses MAPKKK18 activity and promotes MAPKKK18 degradation by the proteasome pathway upon abscisic acid (ABA) treatment. Represses KIN10 activity by the specific dephosphorylation of its T-loop Thr-198, leading to a poststress inactivation of SnRK1 signaling. Restricts MAPKKK20 activity by dephosphorylation.
  • 基因功能參考文獻：
    1. Phosphatase ABI1 and okadaic acid-sensitive phosphatases of the PPP family are negative regulators of salt stress-activated SnRK2.4. PMID: 27297076
    2. ABI1 acts to inhibit MAPKKK18 kinase activity, but also affects MAPKKK18 protein turnover via the ubiquitin-proteasome pathway. SnRK2.6 kinase also seems to be important for the regulation of MAPKKK18 function. PMID: 26852793
    3. ABI1 regulates the activity and promotes proteasomal degradation of MKKK18. PMID: 26443375
    4. The role of ABI1 in the activation of nuclear genes during transcription of chloroplast genes PMID: 25976841
    5. There is direct cross-talk between C/N and non-canonical ABA signalling pathways, regulated by ABI1, in plants. PMID: 25795738
    6. In the endodermis, misexpression of the ABA insensitive1-1 mutant protein, which dominantly inhibits abscisic acid (ABA) signaling, leads to a substantial recovery in lateral root (LR) growth under salt stress conditions. PMID: 23341337
    7. Two dominant-negative mutations in ABA insensitive1 (abi1-1) and abi2-1 greatly reduced ROS production in mitochondria. PMID: 22652060
    8. ROP11 protects ABI1 phosphatase activity from inhibition by RCAR1/PYL9 and thus negatively regulates abscissic acid signaling. PMID: 22251383
    9. the molecular basis for PP2Cs-mediated inhibition of SnRK2s PMID: 22090030
    10. study demonstrates ABI1 can interact with several PYR/PYL/RCAR family members, that PYR1-ABI1 interaction is rapidly stimulated by ABA & indicate new SnRK2 kinase-PYR/PYL/RCAR interactions in an emerging model for PYR/PYL/RCAR-mediated ABA signalling PMID: 19874541
    11. HAB1 and the related PP2Cs ABI1 and ABI2 interact with OST1 in vivo, and mutations in the corresponding genes strongly affect OST1 activation by ABA. PMID: 19855047
    12. The consequences of altered abscisic acid (ABA) sensitivity in gray poplar (Populus x canescens [Ait.] Sm.) development were examined by ectopic expression of the Arabidopsis (Arabidopsis thaliana) mutant abi1 (for abscisic acid insensitive1) gene. PMID: 19837818
    13. the direct interaction between SRK2E/OST1 and ABI1 through Domain II plays a critical role in the control of stomatal closure PMID: 16365038
    14. ABI1 and ABI2 control fibrillin expression that is involved in mediating ABAinduced photoprotection. [ABI1] PMID: 16571665
    15. ABI1 works with HAB1 to negatively regulate ABA signaling. PMID: 16798945
    16. AtBI1 plays a pivotal role as a highly conserved survival factor during ER stress that acts in parallel to the unfolded protein response pathway PMID: 18039663
    17. abi1-1 acts as a hypermorphic allele, and ABI1 reprograms sensitivity towards ABA in the nucleus PMID: 18298671
    18. regulatory component of abscissic acid (ABA)receptor 1(RCAR1, At1g01360) was shown to bind ABA, to mediate ABA-dependent inactivation of type 2C protein phosphatases (PP2Cs) ABI1 or ABI2 in vitro, and to antagonize PP2C action in planta PMID: 19407143
    19. ABI1 plays the role of a general signal transducer linking abscisic acid and ethylene biosynthesis as well as signalling pathways to ozone stress tolerance. PMID: 19705149

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  • 亞細胞定位：
    Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Associated to the plasma membrane when in complex with PA, subsequently to ABA signaling.
  • 蛋白家族：
    PP2C family
  • 組織特異性：
    Expressed in seeds and seedlings. In roots, confined to lateral root caps and columella cells.
  • 數據庫鏈接：

    KEGG: ath:AT4G26080

    STRING: 3702.AT4G26080.1

    UniGene: At.21332



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