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Recombinant Arabidopsis thaliana ATPase 2, plasma membrane-type (AHA2), partial

  • 中文名稱:
    Recombinant Arabidopsis thaliana ATPase 2, plasma membrane-type(AHA2) ,partial
  • 貨號:
    CSB-YP325675DOA
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Arabidopsis thaliana ATPase 2, plasma membrane-type(AHA2) ,partial
  • 貨號:
    CSB-EP325675DOA
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Arabidopsis thaliana ATPase 2, plasma membrane-type(AHA2) ,partial
  • 貨號:
    CSB-EP325675DOA-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Arabidopsis thaliana ATPase 2, plasma membrane-type(AHA2) ,partial
  • 貨號:
    CSB-BP325675DOA
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Arabidopsis thaliana ATPase 2, plasma membrane-type(AHA2) ,partial
  • 貨號:
    CSB-MP325675DOA
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    AHA2
  • Uniprot No.:
  • 別名:
    AHA2; At4g30190; F9N11.40; ATPase 2; plasma membrane-type; EC 7.1.2.1; Proton pump 2
  • 種屬:
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    The plasma membrane H(+) ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses. Involved in maintaining the membrane potential and delta-pH, together forming the plasma membrane protonmotive force (PMF) required for root and hypocotyl elongation and root tropism. Important for root growth and development during different nitrogen regimes. Forms a functional cation-translocating unit with CNGC17 that is activated by PSKR1/BAK1 and possibly other BAK1/RLK complexes.
  • 基因功能參考文獻:
    1. The accumulation of AHA2 in intracellular compartments is consistent with reduced H(+) secretion near the transition zone and the suppression of root growth. PMID: 29042459
    2. the PM proton pump AHA2 (Arabidopsis plasma membrane H(+) -ATPase isoform 2) is important for root growth and development during different nitrogen regimes. PMID: 25382626
    3. AHA2 is activated by lysophospholipids directly via a mechanism involving N-and C- terminal domains. PMID: 25971968
    4. analysis of Arabidopsis thaliana plasma membrane H(+)-ATPase isoform 2 reconstituted into soluble nanoscale lipid bilayers, also termed nanodiscs PMID: 23836891
    5. Asn-106 is important for closure of the proton entrance pathway prior to proton translocation across the membrane in AHA2 PMID: 23420846
    6. ABA has no effect on blue light-induced phosphorylation of the H+-ATPase in ABA-insensitive mutants PMID: 21666226
    7. AHA2 and Na+,K+-ATPase crystals were both highly fragile and sensitive to manipulation, resulting in only low-resolution heavy-atom derivative data sets being available for phasing (6-9 A degrees maximum resolution). PMID: 20179343
    8. first structure of a P-type proton pump (AHA2) determined by X-ray crystallography PMID: 18075595
    9. the rhizosphere acidification in response to Fe deficiency is chiefly mediated by AHA2... PMID: 19549134

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  • 亞細胞定位:
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族:
    Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIIA subfamily
  • 組織特異性:
    Higher levels in roots than in shoots. Expressed in epidermal and root cortex cells, in phloem, xylem and root hairs. Detected in cotyledons, hypocotyls, roots and root hairs.
  • 數據庫鏈接:

    KEGG: ath:AT4G30190

    STRING: 3702.AT4G30190.2

    UniGene: At.23014



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