在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

SARS-CoV-2 Spike RBD Recombinant Nanobody, Biotin conjugated

Rare Species
  • 中文名稱:
    SARS-CoV-2 Spike RBD重組納米抗體, Biotin偶聯
  • 貨號:
    CSB-RA33245D2GMY
  • 規格:
    ¥3080
  • 其他:

產品詳情

  • 產品描述:
    SARS-CoV-2 Spike RBD Nanobody(Biotin標記)是一種特異性靶向新冠病毒刺突蛋白受體結合域(RBD)的重組抗體,其生物素標記特性可適配鏈霉親和素檢測系統,為高靈敏度檢測提供技術基礎。該納米抗體通過識別病毒RBD關鍵表位,能夠阻斷其與宿主ACE2受體的結合,適用于病毒入侵機制研究及中和抗體篩選。經ELISA驗證,該抗體在1:10,000至1:50,000的寬泛稀釋范圍內仍保持優異的信號響應,表明其具備高親和力與穩定性。其小分子量特性可有效降低空間位阻,適用于抗原-抗體相互作用的精細結構分析。科研應用方向包括:病毒顆粒檢測、疫苗候選分子評價、抗病毒藥物開發中的靶標驗證,以及基于RBD蛋白的分子互作研究。產品經大腸桿菌重組表達,批次間一致性高,可滿足高通量篩選需求,為新冠病毒基礎研究與防治工具開發提供可靠試劑。
  • Uniprot No.:
  • 別名:
    S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein)
  • 反應種屬:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 免疫原:
    Recombinant Human Novel Coronavirus Spike glycoprotein(S) (319-541aa) (CSB-YP3324GMY1 and CSB-MP3324GMY1b1)
  • 免疫原種屬:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 標記方式:
    Biotin
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    VHH fusion with human IgG1 Fc
  • 純化方式:
    Affinity-chromatography
  • 克隆號:
    A1
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA
  • 推薦稀釋比:
    Application Recommended Dilution
    ELISA 1:10000-1:50000
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.; May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.
  • 基因功能參考文獻:
    1. Study presents crystal structure of C-terminal domain of SARS-CoV-2 (SARS-CoV-2-CTD) spike S protein in complex with human ACE2 (hACE2); hACE2-binding mode similar overall to that observed for SARS-CoV. However, details at the binding interface show that key residue substitutions in SARS-CoV-2-CTD slightly strengthen the interaction and lead to higher affinity for receptor binding than SARS-CoV receptor-binding domain. PMID: 32378705
    2. crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2 PMID: 32365751
    3. crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 (engineered to facilitate crystallization) in complex with ACE2 PMID: 32320687
    4. Out of the two isolates from India compared to the isolates from Wuhan, China, one was found to harbor a mutation in its receptor-binding domain (RBD) at position 407 where, arginine was replaced by isoleucine. This mutation has been seen to change the secondary structure of the protein at that region and this can potentially alter receptor binding of the virus. PMID: 32275855
    5. Structural modeling of the SARS-CoV-2 spike glycoprotein show similar receptor utilization between SARS-CoV-2 and SARS-CoV, despite a relatively low amino acid similarity in the receptor binding module. Compared to SARS-CoV and all other coronaviruses in Betacoronavirus lineage B, an extended structural loop containing basic amino acids were identified at the interface of the receptor binding (S1) and fusion (S2) domains. PMID: 32245784
    6. crystal structure of CR3022, a neutralizing antibody from a SARS patient, in complex with the receptor-binding domain of the SARS-CoV-2 spike (S) protein to 3.1 A; study provides insight into how SARS-CoV-2 can be targeted by the humoral immune response and revealed a conserved, but cryptic epitope shared between SARS-CoV-2 and SARS-CoV PMID: 32225176
    7. SARS-CoV and SARS-CoV-2 spike proteins have comparable binding affinities achieved by balancing energetics and dynamics. The SARS-CoV-2-ACE2 complex contains a higher number of contacts, a larger interface area, and decreased interface residue fluctuations relative to the SARS-CoV-ACE2 complex. PMID: 32225175
    8. Interaction interface between cat/dog/pangolin/Chinese hamster ACE2 and SARS-CoV/SARS-CoV-2 S protein was simulated through homology modeling. Authors identified that N82 of ACE2 showed closer contact with receptor-binding domain of S protein than human ACE2. PMID: 32221306
    9. SARS-CoV-2 S glycoprotein harbors a furin cleavage site at the boundary between the S1/S2 subunits, which is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs; determined cryo-EM structures of the SARS-CoV-2 S ectodomain trimer. PMID: 32201080
    10. Study demonstrates that SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. PMID: 32155444
    11. The ACE2-B0AT1 complex exists as a dimer of heterodimers. Structural alignment of the RBD-ACE2-B0AT1 ternary complex with the S protein of SARS-CoV-2 suggests that two S protein trimers can simultaneously bind to an ACE2 homodimer. PMID: 32142651
    12. study demonstrated SARS-CoV-2 S protein entry on 293/hACE2 cells is mainly mediated through endocytosis, and PIKfyve, TPC2 and cathepsin L are critical for virus entry; found that SARS-CoV-2 S protein could trigger syncytia in 293/hACE2 cells independent of exogenous protease; there was limited cross-neutralization activity between convalescent sera from SARS and COVID-19 patients PMID: 32132184
    13. study determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation; provided biophysical and structural evidence that the 2019-nCoV S protein binds angiotensin-converting enzyme 2 (ACE2) with higher affinity than does severe acute respiratory syndrome (SARS)-CoV S PMID: 32075877

    顯示更多

    收起更多

  • 亞細胞定位:
    Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein.
  • 蛋白家族:
    Betacoronaviruses spike protein family


主站蜘蛛池模板: 午夜131美女爱做视频| av无码久久久久久不卡网站| 偷偷要色偷偷中文无码| 天堂а√在线地址8中文种子| 又色又爽又黄的视频国内| 国产亚洲精品久久久久久久| 亚洲综合在线另类色区奇米| 丰满少妇作爱视频免费观看| 一区二区伊人久久大杳蕉| 久久天天躁狠狠躁夜夜2019| 精品国产av 无码一区二区三区 | 老汉色老汉首页a亚洲| 国产午夜成人免费看片app| 久久99亚洲精品久久99果| 激情偷乱人成视频在线观看| 人人爽天天碰狠狠添| 精品无码国产自产拍在线观看| 90后极品粉嫩小泬20p| 精品亚洲国产成人a片app| 人妻换着玩又刺激又爽| 男人猛吃奶女人爽视频| 中文字幕乱妇无码av在线| 麻豆视传媒精品av| 欧美老肥熟妇多毛xxxxx| 亚洲愉拍99热成人精品热久久 | 艳妇臀荡乳欲伦69调教视频| 久久婷婷丁香七月色综合 | 中文字幕精品一区二区2021年 | 体验区试看120秒啪啪免费| 欧美午夜精品久久久久免费视| 亚洲欧美日韩中文久久| 亚洲另类欧美小说图片区| 免费啪视频在线观看视频网页| 国产色婷婷亚洲99精品小说| 国产av国片偷人妻麻豆| 狠狠色噜噜狠狠狠8888在| 人妻激情文学| 一区二区三区国产亚洲网站| 日本熟妇人妻xxxxx-欢迎您| 伊人色合天天久久综合网| 熟女人妻一区二区三区免费看|