在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

SARS-CoV-2 Spike RBD Recombinant Nanobody, Biotin conjugated

Rare Species
  • 中文名稱:
    SARS-CoV-2 Spike RBD重組納米抗體, Biotin偶聯
  • 貨號:
    CSB-RA33245D2GMY
  • 規格:
    ¥3080
  • 其他:

產品詳情

  • 產品描述:
    SARS-CoV-2 Spike RBD Nanobody(Biotin標記)是一種特異性靶向新冠病毒刺突蛋白受體結合域(RBD)的重組抗體,其生物素標記特性可適配鏈霉親和素檢測系統,為高靈敏度檢測提供技術基礎。該納米抗體通過識別病毒RBD關鍵表位,能夠阻斷其與宿主ACE2受體的結合,適用于病毒入侵機制研究及中和抗體篩選。經ELISA驗證,該抗體在1:10,000至1:50,000的寬泛稀釋范圍內仍保持優異的信號響應,表明其具備高親和力與穩定性。其小分子量特性可有效降低空間位阻,適用于抗原-抗體相互作用的精細結構分析。科研應用方向包括:病毒顆粒檢測、疫苗候選分子評價、抗病毒藥物開發中的靶標驗證,以及基于RBD蛋白的分子互作研究。產品經大腸桿菌重組表達,批次間一致性高,可滿足高通量篩選需求,為新冠病毒基礎研究與防治工具開發提供可靠試劑。
  • Uniprot No.:
  • 別名:
    S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein)
  • 反應種屬:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 免疫原:
    Recombinant Human Novel Coronavirus Spike glycoprotein(S) (319-541aa) (CSB-YP3324GMY1 and CSB-MP3324GMY1b1)
  • 免疫原種屬:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 標記方式:
    Biotin
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    VHH fusion with human IgG1 Fc
  • 純化方式:
    Affinity-chromatography
  • 克隆號:
    A1
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA
  • 推薦稀釋比:
    Application Recommended Dilution
    ELISA 1:10000-1:50000
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.; May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.
  • 基因功能參考文獻:
    1. Study presents crystal structure of C-terminal domain of SARS-CoV-2 (SARS-CoV-2-CTD) spike S protein in complex with human ACE2 (hACE2); hACE2-binding mode similar overall to that observed for SARS-CoV. However, details at the binding interface show that key residue substitutions in SARS-CoV-2-CTD slightly strengthen the interaction and lead to higher affinity for receptor binding than SARS-CoV receptor-binding domain. PMID: 32378705
    2. crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2 PMID: 32365751
    3. crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 (engineered to facilitate crystallization) in complex with ACE2 PMID: 32320687
    4. Out of the two isolates from India compared to the isolates from Wuhan, China, one was found to harbor a mutation in its receptor-binding domain (RBD) at position 407 where, arginine was replaced by isoleucine. This mutation has been seen to change the secondary structure of the protein at that region and this can potentially alter receptor binding of the virus. PMID: 32275855
    5. Structural modeling of the SARS-CoV-2 spike glycoprotein show similar receptor utilization between SARS-CoV-2 and SARS-CoV, despite a relatively low amino acid similarity in the receptor binding module. Compared to SARS-CoV and all other coronaviruses in Betacoronavirus lineage B, an extended structural loop containing basic amino acids were identified at the interface of the receptor binding (S1) and fusion (S2) domains. PMID: 32245784
    6. crystal structure of CR3022, a neutralizing antibody from a SARS patient, in complex with the receptor-binding domain of the SARS-CoV-2 spike (S) protein to 3.1 A; study provides insight into how SARS-CoV-2 can be targeted by the humoral immune response and revealed a conserved, but cryptic epitope shared between SARS-CoV-2 and SARS-CoV PMID: 32225176
    7. SARS-CoV and SARS-CoV-2 spike proteins have comparable binding affinities achieved by balancing energetics and dynamics. The SARS-CoV-2-ACE2 complex contains a higher number of contacts, a larger interface area, and decreased interface residue fluctuations relative to the SARS-CoV-ACE2 complex. PMID: 32225175
    8. Interaction interface between cat/dog/pangolin/Chinese hamster ACE2 and SARS-CoV/SARS-CoV-2 S protein was simulated through homology modeling. Authors identified that N82 of ACE2 showed closer contact with receptor-binding domain of S protein than human ACE2. PMID: 32221306
    9. SARS-CoV-2 S glycoprotein harbors a furin cleavage site at the boundary between the S1/S2 subunits, which is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs; determined cryo-EM structures of the SARS-CoV-2 S ectodomain trimer. PMID: 32201080
    10. Study demonstrates that SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. PMID: 32155444
    11. The ACE2-B0AT1 complex exists as a dimer of heterodimers. Structural alignment of the RBD-ACE2-B0AT1 ternary complex with the S protein of SARS-CoV-2 suggests that two S protein trimers can simultaneously bind to an ACE2 homodimer. PMID: 32142651
    12. study demonstrated SARS-CoV-2 S protein entry on 293/hACE2 cells is mainly mediated through endocytosis, and PIKfyve, TPC2 and cathepsin L are critical for virus entry; found that SARS-CoV-2 S protein could trigger syncytia in 293/hACE2 cells independent of exogenous protease; there was limited cross-neutralization activity between convalescent sera from SARS and COVID-19 patients PMID: 32132184
    13. study determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation; provided biophysical and structural evidence that the 2019-nCoV S protein binds angiotensin-converting enzyme 2 (ACE2) with higher affinity than does severe acute respiratory syndrome (SARS)-CoV S PMID: 32075877

    顯示更多

    收起更多

  • 亞細胞定位:
    Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein.
  • 蛋白家族:
    Betacoronaviruses spike protein family


主站蜘蛛池模板: 久久久久人妻精品一区三寸 | 小12萝裸体自慰出白浆| 天堂va视频一区二区| 亚洲午夜av久久久精品影院色戒| 国产精品久久久久久福利| 高级会所人妻互换94部分| 久久亚洲精品国产亚洲老地址| 久久视频在线视频精品| 亚洲精品综合一区二区| 精品久久久无码中文字幕| 少妇被粗大的猛烈进出免费视频| 国产初高中生粉嫩无套第一次| 亚洲色成人四虎在线观看| 亚洲乱色熟女一区二区三区丝袜 | 亚洲亚洲人成无码网www| 国产高清国产精品国产专区| 天天做天天摸天天爽天天爱| 国产爆乳无码视频在线观看3| 久久成人国产精品免费| 欧美孕妇xxxx做受欧美88| 久久人搡人人玩人妻精品| 激情内射日本一区二区三区| 久久久受www免费人成| 久久人人妻人人爽人人爽| 在线观看片免费视频无码| 日韩网红少妇无码视频香港 | 色翁荡熄又大又硬又粗又视频图片| 又黄又爽又色视频免费| 亚洲精品无码不卡在线播放he| 日本熟妇乱子伦xxxx| 88国产精品视频一区二区三区 | 国产精品国产av片国产| 一区二三区在线 | 中国| 国产成人综合亚洲看片| 欧美人与动牲交zooz乌克兰| 好紧好爽免费午夜视频| 男人的好看免费观看在线视频| 成人性生交大片免费看中文 | 国产婷婷精品av在线| 久久人人97超碰国产公开| 欧美xxxxx高潮喷水|