在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

S Recombinant Monoclonal Antibody, FITC conjugated

Rare Species
  • 中文名稱:
    S重組抗體, FITC偶聯
  • 貨號:
    CSB-RA33245C1GMY
  • 規格:
    ¥1540
  • 其他:

產品詳情

  • 產品描述:
    CSB-RA33245C1GMY是一種FITC標記的重組單克隆抗體,針對S蛋白靶點開發,適用于ELISA檢測。該抗體通過哺乳動物表達系統制備,具有高度均一的抗原結合特性,經嚴格驗證顯示在ELISA實驗中具備優異檢測靈敏度和特異性,檢測線性范圍跨越三個數量級,批間差異小于8%。靶標S蛋白作為信號傳導關鍵因子,在細胞增殖與分化調控中發揮重要作用,其異常表達與多種病理過程相關。本產品采用優化的FITC偶聯工藝,熒光信號穩定性達48小時以上,兼容常規熒光檢測體系,適用于目標蛋白的定量分析、配體結合研究及功能驗證實驗。經交叉反應測試顯示,與同源家族蛋白無顯著結合,確保檢測特異性。該抗體適用于體外樣本檢測,為分子相互作用機制研究、信號通路解析及靶向藥物開發提供可靠工具,尤其適合需要高靈敏度熒光標記的科研場景。
  • Uniprot No.:
  • 別名:
    S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein)
  • 反應種屬:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 免疫原:
    Recombinant Human Novel Coronavirus Spike glycoprotein (S) (16-685aa) (CSB-MP3324GMY)
  • 免疫原種屬:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 標記方式:
    FITC
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Mouse scFv fusion with human IgG1 Fc
  • 純化方式:
    Affinity-chromatography
  • 克隆號:
    H6
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式:
    Liquid
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.; May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.
  • 基因功能參考文獻:
    1. Study presents crystal structure of C-terminal domain of SARS-CoV-2 (SARS-CoV-2-CTD) spike S protein in complex with human ACE2 (hACE2); hACE2-binding mode similar overall to that observed for SARS-CoV. However, details at the binding interface show that key residue substitutions in SARS-CoV-2-CTD slightly strengthen the interaction and lead to higher affinity for receptor binding than SARS-CoV receptor-binding domain. PMID: 32378705
    2. crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2 PMID: 32365751
    3. crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 (engineered to facilitate crystallization) in complex with ACE2 PMID: 32320687
    4. Out of the two isolates from India compared to the isolates from Wuhan, China, one was found to harbor a mutation in its receptor-binding domain (RBD) at position 407 where, arginine was replaced by isoleucine. This mutation has been seen to change the secondary structure of the protein at that region and this can potentially alter receptor binding of the virus. PMID: 32275855
    5. Structural modeling of the SARS-CoV-2 spike glycoprotein show similar receptor utilization between SARS-CoV-2 and SARS-CoV, despite a relatively low amino acid similarity in the receptor binding module. Compared to SARS-CoV and all other coronaviruses in Betacoronavirus lineage B, an extended structural loop containing basic amino acids were identified at the interface of the receptor binding (S1) and fusion (S2) domains. PMID: 32245784
    6. crystal structure of CR3022, a neutralizing antibody from a SARS patient, in complex with the receptor-binding domain of the SARS-CoV-2 spike (S) protein to 3.1 A; study provides insight into how SARS-CoV-2 can be targeted by the humoral immune response and revealed a conserved, but cryptic epitope shared between SARS-CoV-2 and SARS-CoV PMID: 32225176
    7. SARS-CoV and SARS-CoV-2 spike proteins have comparable binding affinities achieved by balancing energetics and dynamics. The SARS-CoV-2-ACE2 complex contains a higher number of contacts, a larger interface area, and decreased interface residue fluctuations relative to the SARS-CoV-ACE2 complex. PMID: 32225175
    8. Interaction interface between cat/dog/pangolin/Chinese hamster ACE2 and SARS-CoV/SARS-CoV-2 S protein was simulated through homology modeling. Authors identified that N82 of ACE2 showed closer contact with receptor-binding domain of S protein than human ACE2. PMID: 32221306
    9. SARS-CoV-2 S glycoprotein harbors a furin cleavage site at the boundary between the S1/S2 subunits, which is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs; determined cryo-EM structures of the SARS-CoV-2 S ectodomain trimer. PMID: 32201080
    10. Study demonstrates that SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. PMID: 32155444
    11. The ACE2-B0AT1 complex exists as a dimer of heterodimers. Structural alignment of the RBD-ACE2-B0AT1 ternary complex with the S protein of SARS-CoV-2 suggests that two S protein trimers can simultaneously bind to an ACE2 homodimer. PMID: 32142651
    12. study demonstrated SARS-CoV-2 S protein entry on 293/hACE2 cells is mainly mediated through endocytosis, and PIKfyve, TPC2 and cathepsin L are critical for virus entry; found that SARS-CoV-2 S protein could trigger syncytia in 293/hACE2 cells independent of exogenous protease; there was limited cross-neutralization activity between convalescent sera from SARS and COVID-19 patients PMID: 32132184
    13. study determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation; provided biophysical and structural evidence that the 2019-nCoV S protein binds angiotensin-converting enzyme 2 (ACE2) with higher affinity than does severe acute respiratory syndrome (SARS)-CoV S PMID: 32075877

    顯示更多

    收起更多

  • 亞細胞定位:
    Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein.
  • 蛋白家族:
    Betacoronaviruses spike protein family


主站蜘蛛池模板: 亚洲愉拍自拍另类天堂| 又紧又大又爽精品一区二区| 亚洲熟妇久久精品| 免费国产黄网站在线观看视频| s级爆乳玩具酱国产vip皮裤| 制服视频在线一区二区| 日产精品卡2卡三卡乱码网站 | 欲女熟妇国产一区二区| 波多野结衣av在线观看| 一二三四在线视频社区3| 亚洲日本va中文字幕| 天天综合天天爱天天做| 亚洲国产精品高清久久久| 亚洲熟妇av一区二区三区宅男| 777米奇色狠狠俺去啦奇米77| 亚洲综合天堂一区二区三区| 中文字幕精品亚洲人成在线| 夜夜高潮夜夜爽夜夜爱爱一区| 强奷乱码中文字幕熟女一| 中国精品少妇hd| 亚洲精品无码久久千人斩 | 国产精品一区二区av片| 乱人伦中文无码视频| 午夜天堂一区人妻| 国产欧美日韩综合精品二区| 国产私拍大尺度在线视频| 久久五十路丰满熟女中出| 国产一区二区不卡在线看| 国产精品18禁污污网站| 久久99国产综合精品| 国产精品久久毛片av大全日韩| 免费观看的av在线播放| 亚洲国产激情一区二区三区| 国产精品天干在线观看| 农村老熟妇乱子伦视频| 麻豆亚洲一区| 免费又黄又裸乳的视频| 国产成人av国语在线观看| 四虎成人国产精品永久在线| 国产成年无码v片在线| 国产精品久久久久久久久久久不卡|