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TPSB2 Recombinant Monoclonal Antibody

  • 中文名稱：
    TPSB2重組抗體
  • 貨號：
    CSB-RA958088A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Mouse Kidney tissue lysate,
      All lanes: TPSB2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 30 kDa
      Observed band size: 30 kDa
    • Overlay Peak curve showing K562 cells stained with CSB-RA958088A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • 產品描述：
    CSB-RA958088A0HU TPSB2重組單克隆抗體是針對人源類胰蛋白酶β2（Tryptase Beta-2）研發的高特異性檢測工具，適用于多種實驗場景。TPSB2基因編碼的絲氨酸蛋白酶主要儲存于肥大細胞顆粒中，參與調控炎癥反應、組織修復及免疫應答等生理病理過程，其異常表達與過敏性疾病、哮喘等炎癥相關疾病機制密切相關。本抗體經嚴格驗證，在Western Blot中可清晰識別內源性TPSB2蛋白，推薦使用稀釋比例為1:500-1:2000；流式細胞分析（FC）應用中建議稀釋比例為1:50-1:200，同時支持ELISA檢測，展現優異的批次穩定性和抗原結合能力。該產品適用于肥大細胞功能研究、炎癥信號通路解析及疾病模型構建等科研領域，為探索免疫調控機制和藥物靶點篩選提供可靠工具，所有應用僅限于科學研究用途。
  • Uniprot No.：
  • 基因名：
    TPSB2
  • 別名：
    Tryptase beta-2 (Tryptase-2) (EC 3.4.21.59) (Tryptase II), TPSB2, TPS2
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from Human TPSB2
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    9H6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
  • 基因功能參考文獻：
    1. These observations indicate that betaII-tryptase activity is post-translationally regulated by an allosteric disulfide bond. PMID: 24142694
    2. Results indicate a key role for heparin in the activation of human betaI- and beta2-tryptase. PMID: 15567416
    3. The disease severity and plasma tryptase levels were not affected by the number of alpha or beta tryptase alleles in mastocytosis patients. PMID: 17449330
    4. Strong linkage of TPSAB1 and TPSB2 and pairing of deficiency alleles with functional alleles in observed haplotypes protect human subjects from "knockout" genomes and indeed from inheritance of fewer than 2 active alleles. PMID: 19748655
  • 亞細胞定位：
    Secreted. Note=Released from the secretory granules upon mast cell activation.
  • 蛋白家族：
    Peptidase S1 family, Tryptase subfamily
  • 數據庫鏈接：

    HGNC: 14120

    OMIM: 191081

    KEGG: hsa:64499

    UniGene: Hs.405479



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