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  4. Human Tryptase beta-2(TPSB2) ELISA kit

Human Tryptase beta-2(TPSB2) ELISA kit

  • 中文名稱：
    人胰蛋白酶β-2(TPSB2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL024128HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人胰蛋白酶β-2(TPSB2)酶聯(lián)免疫試劑盒（CSB-EL024128HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TPSB2含量。TPSB2（類胰蛋白酶2）是一種胰蛋白酶樣絲氨酸蛋白酶，屬于肽酶家族S1。它僅作為肝素穩(wěn)定的四聚體具有酶促活性，對(duì)所有已知的內(nèi)源性蛋白酶抑制劑具有抗性，并在哮喘和其他過敏性和炎癥性疾病發(fā)病機(jī)制中發(fā)揮作用。試劑盒檢測(cè)范圍為0.137 ng/ml- 100 ng/ml，支持科研領(lǐng)域?qū)PSB2的功能研究，例如炎癥性疾病模型的分子機(jī)制探索、藥物干預(yù)效果評(píng)估或細(xì)胞分泌功能分析，也可用于生物樣本中TPSB2表達(dá)水平的定量比較實(shí)驗(yàn)。適用于生命科學(xué)基礎(chǔ)研究及生物標(biāo)志物開發(fā)等場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Mast cell tryptase beta II ELISA Kit; Mast cell tryptase beta III ELISA Kit; TPS2 ELISA Kit; TPSB2 ELISA Kit; TRYB2_HUMAN ELISA Kit; Tryptase beta 2 (gene/pseudogene) ELISA Kit; Tryptase beta-2 ELISA Kit; Tryptase II ELISA Kit; Tryptase-2 ELISA Kit; TryptaseB ELISA Kit; TryptaseC ELISA Kit
  • 縮寫：
    TPSB2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.137 ng/ml - 100 ng/ml
  • 靈敏度：
    0.137 ng/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TPSB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    92

    Range %

    85-99

    1:2

    Average %

    89

    Range %

    84-95

    1:4

    Average %

    93

    Range %

    89-98

    1:8

    Average %

    90

    Range %

    83-97

  • 回收率：

    The recovery of human TPSB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    86-100

    EDTA plasma (n=4)

    101

    96-107

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    100

    2.576

    2.634

    2.605

    2.502

    33.33

    1.807

    1.767

    1.787

    1.684

    11.11

    1.124

    1.188

    1.156

    1.053

    3.7

    0.639

    0.629

    0.634

    0.531

    1.23

    0.388

    0.371

    0.380

    0.277

    0.41

    0.296

    0.286

    0.291

    0.188

    0.137

    0.185

    0.184

    0.185

    0.082

    0

    0.101

    0.105

    0.103

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
  • 基因功能參考文獻(xiàn)：
    1. These observations indicate that betaII-tryptase activity is post-translationally regulated by an allosteric disulfide bond. PMID: 24142694
    2. Results indicate a key role for heparin in the activation of human betaI- and beta2-tryptase. PMID: 15567416
    3. The disease severity and plasma tryptase levels were not affected by the number of alpha or beta tryptase alleles in mastocytosis patients. PMID: 17449330
    4. Strong linkage of TPSAB1 and TPSB2 and pairing of deficiency alleles with functional alleles in observed haplotypes protect human subjects from "knockout" genomes and indeed from inheritance of fewer than 2 active alleles. PMID: 19748655
  • 亞細(xì)胞定位：
    Secreted. Note=Released from the secretory granules upon mast cell activation.
  • 蛋白家族：
    Peptidase S1 family, Tryptase subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 14120

    OMIM: 191081

    KEGG: hsa:64499

    UniGene: Hs.405479



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