MAGED2 Antibody
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中文名稱：MAGED2兔多克隆抗體
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貨號：CSB-PA891976LA01HU
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規格：￥440
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圖片：
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Western Blot
Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate
All lanes: MAGED2 antibody at 4.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65, 64 kDa
Observed band size: 65, 64 kDa -
IHC image of CSB-PA891976LA01HU diluted at 1:320 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-PA891976LA01HU diluted at 1:320 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of Hela cells with CSB-PA891976LA01HU at 1:145, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunoprecipitating MAGED2 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA891976LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA891976LA01HU (6µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (20µg)
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) MAGED2 Polyclonal antibody
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Uniprot No.：
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基因名：MAGED2
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別名：11B6 antibody; BCG 1 antibody; BCG-1 antibody; Breast cancer-associated gene 1 protein antibody; HCA10 antibody; Hepatocellular carcinoma-associated protein JCL-1 antibody; JCL 1 antibody; MAGD2_HUMAN antibody; MAGE D antibody; MAGE D2 antigen antibody; MAGE-D2 antigen antibody; MAGED2 antibody; Melanoma antigen family D 2 antibody; Melanoma associated antigen D2 antibody; Melanoma-associated antigen D2 antibody
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宿主：Rabbit
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反應種屬：Human
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免疫原：Recombinant Human Melanoma-associated antigen D2 protein (38-285AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產品，MAGED2 Antibody (CSB-PA891976LA01HU)，的標記方式是Non-conjugated。對于MAGED2 Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 IHC 1:200-1:500 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Regulates the expression, localization to the plasma membrane and function of the sodium chloride cotransporters SLC12A1 and SLC12A3, two key components of salt reabsorption in the distal renal tubule.
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基因功能參考文獻：
- Increased expression of MAGE-D2 mRNA was associated with distant metastasis in Gastric Cancer. PMID: 25743330
- MAGE-D2 is a dynamic protein whose shuttling properties could suggest a role in cell cycle regulation. PMID: 26705694
- We found that MAGED2 mutations caused X-linked polyhydramnios with prematurity and a severe but transient form of antenatal Bartter's syndrome. PMID: 27120771
- these results identify the expression of MAGE-D2 suppresses TRAIL receptor 2 and protects againstas TRAIL-induced apoptosis PMID: 22791814
- Expression pattern and further characterization of human MAGED2 and identification of rodent orthologues PMID: 11856887
- Maged2 is mainly expressed in tissues of mesodermal origin PMID: 15162511
- Results identified a cDNA clone, corresponding to MAGE D2 mRNA, from primary human bronchial epithelial cells which exhibits increased expression in vitro after treatment with all-trans retinoic acid. PMID: 15465002
- MAGED2, a novel protein, is a p53-dissociator. PMID: 17912449
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相關疾病：Bartter syndrome 5, antenatal, transient (BARTS5)
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組織特異性：Widely expressed. In the developing and adult kidney, expressed in the thick ascending limb of the loop of Henle and the distal convoluted tubules outside the loop.
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數據庫鏈接：
