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  4. Human Melanoma-associated antigen D2(MAGED2) ELISA kit

Human Melanoma-associated antigen D2(MAGED2) ELISA kit

  • 中文名稱：
    人黑色素瘤相關(guān)抗原D2(MAGED2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL013352HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人黑色素瘤相關(guān)抗原D2(MAGED2)酶聯(lián)免疫試劑盒（CSB-EL013352HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的MAGED2含量。MAGED2基因是MAGE家族成員之一，位于X染色體上，與X連鎖智力障礙和某些癌癥相關(guān)。其蛋白在細(xì)胞周期調(diào)節(jié)中發(fā)揮作用，可變剪接導(dǎo)致多個(gè)轉(zhuǎn)錄本變體。研究發(fā)現(xiàn)，MAGED2蛋白具有抗病毒功能，新冠病毒主蛋白酶可切割MAGED2蛋白，使其失去抗病毒作用。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，本產(chǎn)品可廣泛應(yīng)用于腫瘤分子機(jī)制研究、抗癌藥物體外篩選及腫瘤相關(guān)生物標(biāo)志物的表達(dá)水平分析等科研領(lǐng)域，為探索MAGED2在腫瘤微環(huán)境調(diào)控、轉(zhuǎn)移潛能評(píng)估等方向提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    11B6 ELISA Kit; BCG 1 ELISA Kit; BCG-1 ELISA Kit; Breast cancer-associated gene 1 protein ELISA Kit; HCA10 ELISA Kit; Hepatocellular carcinoma-associated protein JCL-1 ELISA Kit; JCL 1 ELISA Kit; MAGD2_HUMAN ELISA Kit; MAGE D ELISA Kit; MAGE D2 antigen ELISA Kit; MAGE-D2 antigen ELISA Kit; MAGED2 ELISA Kit; Melanoma antigen family D 2 ELISA Kit; Melanoma associated antigen D2 ELISA Kit; Melanoma-associated antigen D2 ELISA Kit
  • 縮寫：
    MAGED2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MAGED2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 83-94
    1:2 Average % 97
    Range % 92-101
    1:4 Average % 93
    Range % 88-97
    1:8 Average % 107
    Range % 102-111
  • 回收率：
    The recovery of human MAGED2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 105 102-108
    EDTA plasma (n=4) 96 91-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.238 2.334 2.286 2.090
    1000 1.415 1.527 1.471 1.275
    500 0.881 0.932 0.907 0.711
    250 0.560 0.573 0.567 0.371
    125 0.379 0.381 0.380 0.184
    62.5 0.292 0.284 0.288 0.092
    31.25 0.247 0.252 0.250 0.054
    0 0.195 0.196 0.196 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Regulates the expression, localization to the plasma membrane and function of the sodium chloride cotransporters SLC12A1 and SLC12A3, two key components of salt reabsorption in the distal renal tubule.
  • 基因功能參考文獻(xiàn)：
    1. Increased expression of MAGE-D2 mRNA was associated with distant metastasis in Gastric Cancer. PMID: 25743330
    2. MAGE-D2 is a dynamic protein whose shuttling properties could suggest a role in cell cycle regulation. PMID: 26705694
    3. We found that MAGED2 mutations caused X-linked polyhydramnios with prematurity and a severe but transient form of antenatal Bartter's syndrome. PMID: 27120771
    4. these results identify the expression of MAGE-D2 suppresses TRAIL receptor 2 and protects againstas TRAIL-induced apoptosis PMID: 22791814
    5. Expression pattern and further characterization of human MAGED2 and identification of rodent orthologues PMID: 11856887
    6. Maged2 is mainly expressed in tissues of mesodermal origin PMID: 15162511
    7. Results identified a cDNA clone, corresponding to MAGE D2 mRNA, from primary human bronchial epithelial cells which exhibits increased expression in vitro after treatment with all-trans retinoic acid. PMID: 15465002
    8. MAGED2, a novel protein, is a p53-dissociator. PMID: 17912449

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  • 相關(guān)疾?。?/div>
    Bartter syndrome 5, antenatal, transient (BARTS5)
  • 組織特異性：
    Widely expressed. In the developing and adult kidney, expressed in the thick ascending limb of the loop of Henle and the distal convoluted tubules outside the loop.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 16353

    OMIM: 300470

    KEGG: hsa:10916

    STRING: 9606.ENSP00000218439

    UniGene: Hs.522665



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