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  4. MCM9 Monoclonal Antibody

MCM9 Monoclonal Antibody

  • 中文名稱：
    MCM9鼠單克隆抗體
  • 貨號：
    CSB-MA882135A0m
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: SY-5Y whole cell lysate, Hela whole cell lysate,U87 whole cell lysate
      All lanes: MCM9 antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 128, 73, 44, 85 kDa
      Observed band size: 128 kDa
      Exposure time：5min
    • IHC image of CSB-MA882135A0m diluted at 1:100 and staining in paraffin-embedded human testes tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

產品詳情

  • 產品描述：
    MCM9單克隆抗體（CUSABIO貨號：CSB-MA882135A0m）是專為人源樣本設計的科研試劑，針對DNA復制與修復關鍵調控因子MCM9蛋白進行精準檢測。作為微染色體維持蛋白家族成員，MCM9在DNA解旋酶復合體組裝及同源重組修復過程中發揮核心作用，其異常表達與基因組不穩定性密切相關。該抗體經嚴格驗證適用于ELISA、Western Blot及免疫組化三大實驗平臺，具有優異的人源特異性反應能力。在基礎研究領域，該產品可助力解析細胞周期調控機制，探索腫瘤發生發展過程中DNA復制應激的分子病理機制，并為生殖細胞發育、干細胞分化等生命過程的分子機理研究提供可靠工具。本產品采用雜交瘤技術制備，確保批次間高質量穩定性，適用于蛋白質相互作用研究、基因表達調控分析及疾病相關信號通路探索等前沿科研場景。通過特異性識別MCM9抗原表位，為研究人員在癌癥生物學、遺傳穩定性研究及抗腫瘤藥物開發等領域提供高靈敏度的檢測解決方案。
  • 產品名稱：
    Mouse anti-Homo sapiens (Human) MCM9 Monoclonal Antibody antibody
  • Uniprot No.：
  • 基因名：
    MCM9
  • 別名：
    MCM9 antibody; C6orf61 antibody; MCMDC1DNA helicase MCM9 antibody; hMCM9 antibody; EC 3.6.4.12 antibody; Mini-chromosome maintenance deficient domain-containing protein 1 antibody; Minichromosome maintenance 9 antibody
  • 宿主：
    Mouse
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human DNA helicase MCM9 protein (1-391AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal Antibody
  • 抗體亞型：
    IgG1
  • 純化方式：
    >95%, Protein G purified
  • 克隆號：
    5H8E10
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand. In addition, recruits MLH1, a component of the MMR complex, to chromatin. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. Probably by regulating HR, plays a key role during gametogenesis.
  • 基因功能參考文獻：
    1. stalled replication forks can be restarted in S phase via homologous recombination using MCM8-9 as an alternative replicative helicase. PMID: 28487407
    2. Significant number of potentially damaging and novel variants in MCM9 in primary ovarian insufficiency; multiallelic association with variants in DDR and MCM8-MCM9 interactome genes. PMID: 27802094
    3. Study identified fifteen variants that included six common SNPs and nine variants of unknown significance (VUS) in MCM9 gene. However VUS occur in MCM9 in a small proportion of Lynch-like syndrome (LLS) patients and MCM9 mutations are unlikely to explain most LLS cases. PMID: 27886675
    4. Data show that the two affected sisters were homozygous for the mutation of MCM9 gene, encoding the minichromosome maintenance complex component 9. PMID: 26771056
    5. MCM9 loading onto chromatin is MSH2-dependent, and in turn MCM9 stimulates the recruitment of MLH1 to chromatin, revealing a role for MCM9 and its helicase activity in DNA mismatch repair. PMID: 26300262
    6. Autosomal-recessive variants in MCM9 cause a genomic-instability syndrome associated with hypergonadotropic hypogonadism and short stature. PMID: 25480036
    7. Chromatin immunoprecipitation analysis using human DR-GFP cells demonstrated that MCM8 and MCM9 proteins are rapidly recruited to DNA damage sites and promote RAD51 recruitment. PMID: 23401855
    8. A novel alternatively spliced variant of MCM9 is specifically induced after exposure to Mitomycin C. Expression is cell-cycle regulated and induced in S-phase. PMID: 23403237
    9. Identified a novel MCM family gene, MCM9, by using bioinformatics; mouse MCM9 mRNA was upregulated by transcription factor E2E1 and serum stimulation in NIH3T3 cells. PMID: 15850810
    10. We also show that the very recently reported human MCM9 protein (HsMCM9), which resembles a truncated MCM-like protein missing a part of the MCM2-7 signature domain, is an incomplete form of the full length HsMCM9 described here. PMID: 16226853
    11. Cdt1, with its two opposing regulatory binding factors MCM9 and geminin, appears to be a major platform on the pre-replication complexes to integrate cell-cycle signals. PMID: 18657502

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  • 相關疾病：
    Ovarian dysgenesis 4 (ODG4)
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    MCM family
  • 數據庫鏈接：

    HGNC: 21484

    OMIM: 610098

    KEGG: hsa:254394

    STRING: 9606.ENSP00000314505

    UniGene: Hs.279008



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