Rat tissue factor(TF)/coagulation factor VII(F7) complex ELISA kit
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中文名稱(chēng):大鼠組織因子與活化凝血因子7復(fù)合物酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EQ027252RA
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠組織因子與活化凝血因子7復(fù)合物酶聯(lián)免疫試劑盒(CSB-EQ027252RA)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的TF含量。TF即組織因子,是一種跨膜糖蛋白。它是外源性凝血途徑的啟動(dòng)因子,在生理止血中起關(guān)鍵作用。研究發(fā)現(xiàn)TF還參與腫瘤生長(zhǎng)、轉(zhuǎn)移及血管生成等。其作用機(jī)制主要是通過(guò)與凝血因子結(jié)合,啟動(dòng)凝血級(jí)聯(lián)反應(yīng)并激活相關(guān)信號(hào)通路。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL,適用于心血管疾病、血栓性疾病等動(dòng)物模型的機(jī)制研究,或抗凝藥物、靶向治療方案的體外效果評(píng)估。為凝血相關(guān)基礎(chǔ)研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě):
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種屬:Rattus norvegicus (Rat)
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樣本類(lèi)型:serum, plasma, tissue homogenates
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檢測(cè)范圍:15.6 pg/mL-1000 pg/mL
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靈敏度:3.9 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cardiovascular
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat TF/F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 107 Range % 98-110 1:2 Average % 103 Range % 96-107 1:4 Average % 98 Range % 89-102 1:8 Average % 105 Range % 98-110 -
回收率:
The recovery of rat TF/F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 85-96 EDTA plasma (n=4) 102 95-107 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1000 2.708 2.673 2.691 2.589 500 2.243 2.150 2.197 2.095 250 1.419 1.469 1.444 1.342 125 0.702 0.734 0.718 0.616 62.5 0.412 0.425 0.419 0.317 31.2 0.251 0.263 0.257 0.155 15.6 0.167 0.172 0.170 0.068 0 0.101 0.103 0.102 -
數(shù)據(jù)處理:
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貨期:3-5 working days
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