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  4. Rat Transferrin(TF) ELISA Kit

Rat Transferrin(TF) ELISA Kit

  • 中文名稱：
    大鼠轉鐵蛋白(TF)酶聯免疫試劑盒
  • 貨號：
    CSB-E12723r
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠轉鐵蛋白(TF)酶聯免疫試劑盒（CSB-E12723r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TF含量。TF即組織因子，是一種跨膜糖蛋白。它是凝血系統的重要啟動因子，在血管損傷時啟動外源性凝血途徑。研究機制主要圍繞其在凝血級聯反應中的作用展開，同時在炎癥、腫瘤轉移等病理過程中也有重要作用，是潛在的治療靶點。試劑盒檢測范圍為7.8 pmol/mL-500 pmol/mL，廣泛應用于鐵代謝研究、肝臟功能評估、營養干預實驗以及疾病動物模型（如貧血、慢性炎癥或鐵過載相關病理）的機制探索，為體外實驗提供可靠的生物標志物檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Tf ELISA Kit; Serotransferrin ELISA Kit; Transferrin ELISA Kit; Beta-1 metal-binding globulin ELISA Kit; Liver regeneration-related protein LRRG03 ELISA Kit; Siderophilin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 pmol/mL-500 pmol/mL
  • 靈敏度：
    1.95 pmol/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %89
    Range %85-92
    1:200Average %96
    Range %89-104
    1:400Average %90
    Range %84-96
    1:800Average %93
    Range %87-99
  • 回收率：
    The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9689-98
    EDTA plasma (n=4)9489-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/mlOD1OD2AverageCorrected
    5002.042 2.143 2.093 1.958
    2501.682 1.696 1.689 1.554
    1251.197 1.234 1.216 1.081
    62.50.803 0.819 0.811 0.676
    31.20.392 0.407 0.400 0.265
    15.60.260 0.298 0.279 0.144
    7.80.173 0.180 0.177 0.042
    00.134 0.136 0.135 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 基因功能參考文獻：
    1. our data establish that blood TSAT exerts a critical role in experimental stroke-induced brain damage. In addition, our findings suggest that the protective effect of iron-free transferrin (apotransferrin, ATf) at the neuronal level resides in preventing NMDA-induced HTf uptake and ROS production, which in turn reduces neuronal damage PMID: 29248829
    2. In brain interstitial fluid, transferrin-bound iron and non-transferrin-bound iron (NTBI) exist as potential iron sources PMID: 25649872
    3. Transferrin and thyroid hormone converge to regulate oligodendrocyte maturation and myelinogenesis. PMID: 25595122
    4. This evidence supports a key role of Tf on the generation of oligodendrocytes (OLs) from Neural stem and progenitor cells (NSC/NPCs)and highlights its potential in demyelinating disorder treatment. PMID: 22479482
    5. Transferrin is involved in the uptake of the radiopharmaceutical gallium-67 (67Ga) by regenerating liver tissue following partial hepatectomy but is not involved in the entry of 67Ga into hepatocytes. PMID: 11767098
    6. data show that brain cells have the capacity to maintain minimum iron levels during iron deficiency which may be associated with increased iron-Tf uptake, stabilization of TfR mRNA, or increased Tf mRNA translation efficiency in specific cell types PMID: 12608731
    7. effects of apotransferrin occur early in development during a narrow, transient "temporal window" within which oligodendroglia are sensitive to its action. PMID: 12730961
    8. first report describing presence of serotransferrin precursor protein homolog belonging to transferrin family and sharing epitopes with melanotransferrin in adrenal, its induction by ACTH, and sensitivity to ginkgolide B (adrenotransferrin). PMID: 14701678
    9. compared to transferrin, soluble melanotransferrin plays little or no role in Fe supply to the brain and erythropoietic tissue PMID: 15469901
    10. in addition to hemoglobin-bound iron, Tf-bound iron may contribute to intracerebral hemorrhage-induced brain injury and thrombin may contribute to the latter by facilitating cellular iron uptake PMID: 15637325
    11. Our studies suggest that AEC I is not just a simple barrier for gas exchange, but a functional cell that protects alveolar epithelium from injury. PMID: 16497717
    12. Plays role in maturation of Schwann cells(SC) in physiological conditions as well as in role in facilitating clearance of myelin debris and subsequent nerve regeneration and/or in SC phenotypic change, which led to survival after peripheral nerve injury. PMID: 17628542
    13. Apotransferrin added to oligodendroglial cell cultures can correct the effects of iron deficiency. PMID: 18459135
    14. serotransferrin levels were increased in diestrous as compared with proestrous, estrous, metestrous PMID: 18511206
    15. we have discovered a novel mitochondrial iron transport system that goes awry in Parkinson's disease, and which may provide a new target for therapeutic intervention. PMID: 19250966
    16. synthesis and secretion of transferrin in rat parotid acinar cells PMID: 19464997

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Transferrin family
  • 組織特異性：
    Expressed by the liver and secreted in plasma.
  • 數據庫鏈接：


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