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  4. Mouse Serotransferrin(TF) ELISA kit

Mouse Serotransferrin(TF) ELISA kit

  • 中文名稱：
    小鼠血清轉鐵蛋白(TF)酶聯免疫試劑盒
  • 貨號：
    CSB-EL023412MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠血清轉鐵蛋白(TF)酶聯免疫試劑盒（CSB-EL023412MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的TF含量。TF即組織因子，是一種跨膜糖蛋白，作為凝血因子Ⅶ/Ⅶa的細胞膜受體。在生理狀態下，TF與血液直接接觸機會少。研究機制上，它可啟動外源性凝血途徑，在止血和血栓形成中起關鍵作用，也參與多種病理過程如炎癥、腫瘤轉移等。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，廣泛應用于基礎研究領域，例如探究鐵代謝相關疾病模型、基因修飾小鼠的表型分析、藥物干預對鐵轉運的影響，或作為血液學、營養學及肝臟功能研究的實驗工具，為科研人員提供高質量的檢測數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Tf ELISA Kit; Trf ELISA Kit; Serotransferrin ELISA Kit; Transferrin ELISA Kit; Beta-1 metal-binding globulin ELISA Kit; Siderophilin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    15.6 ng/mL-1000 ng/mL
  • 靈敏度：
    3.9 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:2000

    Average %

    96

    Range %

    91-103

    1:4000

    Average %

    87

    Range %

    83-93

    1:8000

    Average %

    95

    Range %

    88-101

    1:16000

    Average %

    93

    Range %

    86-99

  • 回收率：

    The recovery of mouse TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    99

    94-103

    EDTA plasma (n=4)

    92

    85-99

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.968

    2.794

    2.881

    2.727

    500

    2.551

    2.467

    2.509

    2.355

    250

    2.047

    2.028

    2.038

    1.883

    125

    1.466

    1.529

    1.498

    1.343

    62.5

    0.916

    0.921

    0.918

    0.764

    31.2

    0.592

    0.613

    0.602

    0.448

    15.6

    0.381

    0.374

    0.378

    0.224

    0

    0.153

    0.155

    0.154

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 基因功能參考文獻：
    1. the value of transferrin receptors (TfRs)/cell reveals a 100-fold increase in the number of TfRs per bEnd3 cells compared to human umbilical vein cells. PMID: 27100851
    2. Identification of multiple transferrin species in the spleen and serum from mice with collagen-induced arthritis which may reflect changes in transferrin glycosylation associated with disease activity: The role of CD38. PMID: 26639305
    3. Transcytosis and brain uptake of transferrin-containing nanoparticles by tuning avidity to transferrin receptor. PMID: 23650374
    4. Together, these data indicate that Lcn2, in combination with transferrin, confines Klebsiella pneumoniae to the airways and prevents invasion into tissue containing the pulmonary vasculature. PMID: 23169997
    5. Transferrin(hpx/hpx) Hemojuvelin(+/+) and Transferrin(hpx/hpx) Hemojuvelin(-/-) phenotypes did not differ markedly, but RBC transfusions increased hepcidin levels in Transferrin(hpx/hpx) Hemojuvelin(+/+) but not Transferrin(hpx/hpx) Hemojuvelin(-/-)mice. PMID: 21993681
    6. These results indicate that CALM is required for erythroid maturation and transferrin internalization in mice. PMID: 22363754
    7. Neurodegenerative conditions involving iron-mediated toxicity may be due to a failure of iron transport or storage mechanisms, rather than to the presence of high levels of non-transferrin-bound iron. PMID: 20431983
    8. Apotransferrin mitigates the neurotoxicity of hemoglobin in a cell culture model of intracerebral hemorrhage. PMID: 21034753
    9. Descibed is the 2.4 A structure of inhibitor of carbonic anhydrase from a pseudomerohedral twinned crystal: a member of the transferrin superfamily. PMID: 20572014
    10. transferrin receptor 2 and HFE are involved in holotransferrin-dependent signaling for the regulation of furin which involved Erk phosphorylation. Furin in turn may control hepcidin expression. PMID: 20634490
    11. hepcidin expression in hypotransferrinemic mice is regulated both by transferrin-restricted erythropoiesis and by transferrin through a mechanism independent of its role in erythropoiesis PMID: 20956801
    12. These data show that mycobacteria reside in the Rab11(+) endocytic recycling pathway, thereby retaining access to metabolizing transferrin and avoiding endocytosed immunoproteins like Lcn2. PMID: 20121435
    13. during later times postinfection (1.5 to 3 h), the virus meets up with transferrin in the Rab11-positive recycling endosome PMID: 16611921
    14. Transsferrin overexpression has negative effects on testicular function and that transfserrin levels require strict regulation in the testis. PMID: 16998850
    15. developing erythroid cells, which are taking up vast amounts of Fe, deliver the metal directly from transferrin-containing endosomes to mitochondria PMID: 17376890
    16. The role of transferrin in glial protection was investigated. PMID: 18509548
    17. Data show that the transferrin receptor TfR1 provided transferrin to the ureteric bud, but not to the capsule or the stroma, and that Scara5 bound serum ferritin and then stimulated its endocytosis from the cell surface with consequent iron delivery. PMID: 19154717

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Transferrin family
  • 組織特異性：
    Expressed by the liver and secreted in plasma.
  • 數據庫鏈接：


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