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  4. Human Protein notum homolog(NOTUM) ELISA kit

Human Protein notum homolog(NOTUM) ELISA kit

  • 中文名稱：
    人notum蛋白同源物(NOTUM)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL015955HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人notum蛋白同源物(NOTUM)酶聯(lián)免疫試劑盒（CSB-EL015955HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NOTUM含量。NOTUM作為一個靶點(diǎn)，它是羧基酯酶家族成員。在研究機(jī)制上，其可通過去除Wnt蛋白的棕櫚酰基抑制Wnt信號通路。在組織發(fā)育、干細(xì)胞維持等生理過程以及腫瘤、神經(jīng)退行性疾病等病理進(jìn)程中發(fā)揮作用，是潛在治療靶點(diǎn)。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，在組織再生、脂肪代謝及神經(jīng)退行性疾病研究中具有重要科研價(jià)值。適用于探究NOTUM在代謝性疾病、腫瘤微環(huán)境調(diào)控或神經(jīng)發(fā)育相關(guān)機(jī)制中的表達(dá)變化，為體外實(shí)驗(yàn)研究提供可靠的蛋白定量支持，滿足基礎(chǔ)科研中對Wnt通路相關(guān)分子調(diào)控機(jī)制的研究需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    NOTUM ELISA Kit; notum pectinacetylesterase homolog (Drosophila) ELISA Kit; Notum pectinacetylesterase homolog ELISA Kit; NOTUM_HUMAN ELISA Kit; Protein notum homolog ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %96
    Range %90-100
    1:2Average %89
    Range %85-93
    1:4Average %92
    Range %88-96
    1:8Average %85
    Range %80-90
  • 回收率：
    The recovery of human NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-100
    EDTA plasma (n=4)106100-114
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2AverageCorrected
    1002.607 2.497 2.552 2.378
    502.203 2.075 2.139 1.965
    251.635 1.542 1.589 1.415
    12.50.952 1.006 0.979 0.805
    6.250.610 0.587 0.599 0.425
    3.120.451 0.462 0.457 0.283
    1.560.332 0.315 0.324 0.150
    00.176 0.171 0.174
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
  • 基因功能參考文獻(xiàn)：
    1. NOTUM expression was increased in metastatic cells. Proliferation was suppressed by inhibiting expression of NOTUM. Knockdown of NOTUM genes inhibited proliferation as well as migration, with possible involvement of p38 and c-JUN N-terminal kinase in this process. PMID: 30343282
    2. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
    3. Kinetic and mass spectrometric analyses of human proteins show that Notum is a carboxylesterase that removes an essential palmitoleate moiety from Wnt proteins and thus constitutes the first known extracellular protein deacylase PMID: 25731175
    4. Overexpression of NOTUM is associated with hepatocellular carcinoma PMID: 18429952
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Pectinacetylesterase family, Notum subfamily
  • 組織特異性：
    Rarely expressed in adult normal tissues.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 27106

    OMIM: 609847

    KEGG: hsa:147111

    STRING: 9606.ENSP00000387310

    UniGene: Hs.106137



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