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  4. Human Lipolysis-stimulated lipoprotein receptor(LSR) ELISA kit

Human Lipolysis-stimulated lipoprotein receptor(LSR) ELISA kit

  • 中文名稱：
    人脂解激活脂蛋白受體(LSR)酶聯免疫試劑盒
  • 貨號：
    CSB-EL013215HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人脂解激活脂蛋白受體(LSR)酶聯免疫試劑盒（CSB-EL013215HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LSR含量。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，本試劑盒通過特異性抗體對捕獲目標蛋白，適用于體外檢測生物樣本中LSR的濃度變化，為研究脂質代謝調控、動脈粥樣硬化形成、肥胖相關分子機制等科研領域提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    ILDR3 ELISA Kit; Immunoglobulin like domain containing receptor 3 ELISA Kit; Lipolysis Stimulated Lipoprotein Receptor ELISA Kit; Lipolysis stimulated remnant ELISA Kit; Lipolysis-stimulated lipoprotein receptor ELISA Kit; LISCH ELISA Kit; LISCH protein ELISA Kit; LISCH7 ELISA Kit; Liver specific bHLH Zip transcription factor ELISA Kit; LSR ELISA Kit; LSR_HUMAN ELISA Kit; MGC10659 ELISA Kit; MGC48312 ELISA Kit; MGC48503 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LSR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %99
    Range %90-103
    1:2Average %96
    Range %90-99
    1:4Average %91
    Range %85-95
    1:8Average %92
    Range %84-97
  • 回收率：
    The recovery of human LSR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9383-97
    EDTA plasma (n=4)10191-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.804 2.722 2.763 2.593
    2002.201 2.129 2.165 1.995
    1001.470 1.442 1.456 1.286
    500.770 0.756 0.763 0.593
    250.500 0.512 0.506 0.336
    12.50.304 0.300 0.302 0.132
    6.250.227 0.235 0.231 0.061
    00.168 0.172 0.170
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells.
  • 基因功能參考文獻：
    1. this study shows that loss of tricellular tight junction protein LSR promotes cell invasion and migration via upregulation of TEAD1/AREG in endometrial cancer PMID: 28071680
    2. Lipolysis-stimulated lipoprotein receptor (LSR)-deficient CaCo-2 cells exhibited increased cell proliferation. PMID: 27391068
    3. Results of this study provide novel evidence that tricellular tight junctions protein LSR negatively regulates cancer cell progression and development in endometrial cancer. PMID: 27036040
    4. Lipolysis-stimulated lipoprotein receptors (LSRs) localized to bicellular junctions in association with myosin regulatory light chain 2 (MRLC2) at low cell densities and to tricellular contacts when myosin phosphatase target subunit 1 (MYPT1) localized to the bicellular regions. PMID: 28493278
    5. analysis of how Clostridium difficile binary toxin CDT interacts with its host cell receptor, Lipolysis-stimulated Lipoprotein Receptor PMID: 25882847
    6. These data show that Clostridium difficile binary toxin CDT induces clustering of the lipolysis-stimulated lipoprotein receptor into lipid rafts. PMID: 23631918
    7. findings show that Clostridium spiroforme toxin (CST)enters target cells via the lipolysis-stimulated lipoprotein receptor; findings indicate that CST shares LSR with C. difficile CDT and C. perfringens iota toxin as a host cell surface receptor PMID: 22252869
    8. LSR defines tricellular contacts in epithelial cellular sheets by acting as a landmark to recruit tricellulin for tricellular tight junction formation. PMID: 21245199
    9. LISCH7 may have a role in progression of human colon cancer PMID: 17975147

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  • 亞細胞定位：
    Cell membrane; Single-pass membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, LISCH7 family
  • 數據庫鏈接：

    HGNC: 29572

    OMIM: 616582

    KEGG: hsa:51599

    STRING: 9606.ENSP00000354575

    UniGene: Hs.466507



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