在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human Leukocyte elastase inhibitor(SERPINB1) ELISA kit

  • 中文名稱:
    人白細胞彈性蛋白酶抑制劑(SERPINB1)酶聯免疫試劑盒
  • 貨號:
    CSB-EL021065HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人白細胞彈性蛋白酶抑制劑(SERPINB1)酶聯免疫試劑盒(CSB-EL021065HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的SERPINB1含量。SERPINB1是一個重要靶點。它屬于絲氨酸蛋白酶抑制劑家族,在細胞內發揮調節蛋白酶活性的作用。研究發現其與免疫調節、炎癥反應等機制相關,對維持細胞內環境穩定有重要意義,有可能成為治療炎癥、腫瘤等疾病的新方向。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,適用于體外研究炎癥相關疾病模型、免疫調控機制探索及細胞功能實驗中目標蛋白的動態分析;支持科研人員開展基礎研究中的蛋白表達量檢測、藥物干預效果評估或病理生理機制解析;為探索SERPINB1在先天免疫、組織修復等領域的生物學功能提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Anti elastase ELISA Kit; EI ELISA Kit; ELANH2 ELISA Kit; Epididymis luminal protein 57 ELISA Kit; HEL57 ELISA Kit; ILEU_HUMAN ELISA Kit; LEI ELISA Kit; Leukocyte elastase inhibitor ELISA Kit; M/NEI ELISA Kit; MNEI ELISA Kit; Monocyte/neutrophil elastase inhibitor ELISA Kit; Peptidase inhibitor 2 ELISA Kit; Pi-2 ELISA Kit; PI2 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1 ELISA Kit; Serpin B1 ELISA Kit; Serpin peptidase inhibitor clade B (ovalbumin) member 1 ELISA Kit; serpinb1 ELISA Kit
  • 縮寫:
    SERPINB1
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %88-101
    1:2Average %93
    Range %84-97
    1:4Average %91
    Range %85-95
    1:8Average %98
    Range %90-101
  • 回收率:
    The recovery of human SERPINB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10294-107
    EDTA plasma (n=4)9890-102
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    401.768 1.700 1.734 1.606
    201.409 1.434 1.422 1.294
    100.886 0.944 0.915 0.787
    50.565 0.551 0.558 0.430
    2.50.379 0.363 0.371 0.243
    1.250.245 0.252 0.249 0.121
    0.6250.201 0.197 0.199 0.071
    00.130 0.126 0.128
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis. Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity. During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. When secreted, promotes the proliferation of beta-cells via its protease inhibitory function.
  • 基因功能參考文獻:
    1. The expression of SERPINB1 was approximately 2-fold higher in apical periodontitis. SERPINB1 expression was noted in neutrophils and epithelial cells. PMID: 28673495
    2. SERPINB1 decreased inflammation, ameliorated oxidative stress in the lung, and attenuated acute lung injury in rats with orthotopic autologous liver transplantation by activating HO-1 and it does so through STAT3 PMID: 28427999
    3. a relatively low Serpinb1a protein threshold in neutrophils that is required for sustained survival PMID: 27107834
    4. SERPINB1 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    5. Data show that high serpin B1 protein (SERPINB1) gene expression was associated with favorable tumor response and prolonged survival under cisplatin-based chemotherapy. PMID: 26799424
    6. Data show that oropharyngeal squamous cell carcinomas (OPSCCs) express granzyme inhibitors SERPINB1, SERPINB4 and SERPINB9 for cytotoxicity and the expression was not different between human papillomavirus (HPV)-positive and HPV-negative tumors. PMID: 26993499
    7. Pediatric CNS-PNETs evade immune recognition by downregulating cell surface MHC-I and CD1d expression. Intriguingly, expression of SERPINB9, SERPINB1, and SERPINB4 is acquired during tumorigenesis in 29%, 29%, and 57% of the tumors PMID: 26963506
    8. is not expressed in neutrophils of both sulfur mustard-exposed and chronic obstructive pulmonary disease patients PMID: 24852194
    9. Data suggest that serine protease inhibitor (SERPIN) B1 negatively regulates glioma cell migration and invasion probably by abrogating the expression of matrix metalloproteinase-2 and the activation of focal adhesion kinase. PMID: 24968089
    10. Decreased expression of SERPINB1 correlates with tumor invasion and poor prognosis in hepatocellular carcinoma. PMID: 24105272
    11. Apoptosis-inducing factor and leukocyte elastase inhibitor derived DNase II interact and can cooperate to induce cell death. PMID: 23673989
    12. Upon reactive center loop cleavage at Phe-343,SERPINB1 covalently complexes with GzmH. SERPINB1 overexpression suppresses GzmH- or LAK cell-mediated cytotoxicity. Crystal structures show possible conformational changes in GzmH for the suicide inhibition. PMID: 23269243
    13. In the resting state during human neutrophil extracellar trap generation, SerpinB1 is exclusively in the cytoplasm, consistent with the current understanding of clade B serpins, and it may migrate and regulate events in the cell nucleus. PMID: 23002442
    14. These results suggest that serpin B1 may be a novel marker of active ulcerative colitis and may play an important role in the pathogenesis of inflammatory bowel disease. PMID: 22421620
    15. serpinB1 sustains a healthy neutrophil reserve that is required in acute immune responses. PMID: 21248149
    16. The findings define an innate role for SerpinB1 in cystic fibrosis airways. PMID: 20817705
    17. M/NEI is a dual specificity inhibitor with two adjacent reactive sites that support rapid efficient inhibitory reactions with cellular proteases, including the three neutrophil granule proteases. PMID: 11747453

    顯示更多

    收起更多

  • 亞細胞定位:
    Secreted. Cytoplasm. Cytolytic granule. Early endosome.
  • 蛋白家族:
    Serpin family, Ov-serpin subfamily
  • 組織特異性:
    In human bone marrow, present in all CD45+ populations. Expression levels are highest in the neutrophil lineage, intermediate in monocytic, and lowest in lymphocytic lineage. Within the neutrophil lineage, expression is highest in promyelocytes.
  • 數據庫鏈接:

    HGNC: 3311

    OMIM: 130135

    KEGG: hsa:1992

    STRING: 9606.ENSP00000370115

    UniGene: Hs.381167



主站蜘蛛池模板: 性高湖久久久久久久久aaaaa| 又色又爽又黄的视频国内| 极品少妇被猛得白浆直流草莓视频| 欧美成人看片黄a免费看| 亚洲精品国产嫩草在线观看东京热 | 中文字幕在线日亚州9| 国产色视频自在线观看| 国产午夜成人无码免费| 中文字幕精品一二三四五六七八 | 国产99视频精品免费视频36| 国产精品亚洲а∨无码播放不卡| 毛片内射久久久一区| 精品久久久无码中文字幕边打电话| 国产α片免费观看在线人| 女人夜夜春高潮爽a∨片| 97高清国语自产拍| 精品国精品国产自在久国产87| 亚洲综合另类小说色区| 国产亚洲精品久久久久久禁果tv| 午夜理论片yy6080私人影院| 国产成人亚洲综合色婷婷| 国产av区男人的天堂| 国语自产精品视频在线第100页| 国产 | 久你欧洲野花视频欧洲1| 亚洲精品字幕| 久久人人爽av亚洲精品| 西西人体444www高清大胆| 日本少妇裸体做爰高潮片| 国产精品嫩草影院久久| 成在人线无码aⅴ免费视频| 亚洲精品天堂成人片av在线播放| 国产a国产片国产| 日韩内射美女片在线观看网站| 丰满熟妇乱子伦| 国产麻豆成人精品av| 精品午夜国产福利观看| 韩国精品视频一区二区在线播放| 国产av无码专区亚洲a∨毛片| 日本三级香港三级人妇三| 色老99久久精品偷偷鲁| 国产免费艾彩sm调教视频|