Horse Hepcidin(HAMP) ELISA kit
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中文名稱:馬鐵調素(HAMP)酶聯免疫試劑盒
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貨號:CSB-EL010124HO
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:馬鐵調素(HAMP)酶聯免疫試劑盒(CSB-EL010124HO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的HAMP含量。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL,適用于科研領域中對鐵代謝調控機制的研究,如探究貧血、鐵代謝紊亂疾病的分子機制,評估動物模型或細胞實驗中鐵調素表達水平的變化,以及相關藥物干預對鐵穩態影響的體外分析。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR, liver-expressed antimicrobial peptide|putative liver tumor regressor
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縮寫:HAMP
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種屬:Equus caballus (Horse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:7.8 ng/mL-500 ng/mL
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靈敏度:1.95 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of horse HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-95 1:2 Average % 102 Range % 97-107 1:4 Average % 91 Range % 85-97 1:8 Average % 97 Range % 91-103 -
回收率:
The recovery of horse HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-98 EDTA plasma (n=4) 97 90-100 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 500 2.556 2.530 2.543 2.441 250 2.065 2.100 2.083 1.981 125 1.621 1.569 1.595 1.493 62.5 1.078 1.098 1.088 0.986 31.2 0.676 0.698 0.687 0.585 15.6 0.377 0.398 0.388 0.286 7.8 0.230 0.244 0.237 0.135 0 0.101 0.102 0.102 -
數據處理:
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貨期:3-5 working days
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