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USP4

USP4,即泛素特異性蛋白酶4,也稱為UNP、UNPH。USP4由人類USP4基因編碼,該基因定位于染色體3的3p21.31區(qū)域。USP4是一種去泛素化酶,屬于半胱氨酸蛋白酶家族,能夠從目標(biāo)蛋白質(zhì)上去除泛素化修飾,調(diào)節(jié)蛋白質(zhì)的穩(wěn)定性和功能。
USP4含有DUSP-UBL結(jié)構(gòu)域和一個(gè)UBL插入催化結(jié)構(gòu)域,與其同源蛋白USP11和USP15共享相同的結(jié)構(gòu)域組織。USP4在多種細(xì)胞和生物學(xué)過程中發(fā)揮關(guān)鍵作用,包括維持內(nèi)質(zhì)網(wǎng)的操作保真度、調(diào)節(jié)細(xì)胞增殖和發(fā)育以及固有免疫的關(guān)鍵途徑。當(dāng)USP4的表達(dá)或活性異常時(shí),它與多種病理狀態(tài)的進(jìn)展有關(guān),尤其是癌癥。

熱銷產(chǎn)品

USP4 Antibody (CSB-PA614393LA01HU)

驗(yàn)證數(shù)據(jù)

CSB-PA614393LA01HU

Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: USP4 antibody at 6.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 109, 104, 36 kDa
Observed band size: 109 kDa

CSB-PA614393LA01HU

IHC image of CSB-PA614393LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-PA614393LA01HU

IHC image of CSB-PA614393LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-PA614393LA01HU

Immunofluorescence staining of U251 cells with CSB-PA614393LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

USP4 Antibodies

USP4 for Homo sapiens (Human)

USP4 Proteins

USP4 Proteins for Homo sapiens (Human)



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