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MS4A1

MS4A1基因編碼CD20蛋白,該抗原為一種疏水性4次跨膜蛋白,分子量大約為35kDa。CD20也叫B淋巴細(xì)胞表面抗原B1、Bp35、白細(xì)胞表面抗原Leu-16等等。CD20只在B淋巴細(xì)胞上表達(dá),參與調(diào)節(jié)B細(xì)胞活化和增殖,作為鈣離子通道發(fā)揮作用。除在正常B細(xì)胞中表達(dá)外,CD20還在B細(xì)胞來(lái)源的淋巴瘤、白血病等的腫瘤細(xì)胞表達(dá),以及涉及免疫疾病和炎癥疾病的B細(xì)胞中表達(dá),所以CD20抗原成為淋巴癌、白血病和某些自體免疫等疾病治療的目標(biāo)靶點(diǎn)。目前,針對(duì)這些疾病的CD20治療主要有三種方式,包括抗CD20單克隆抗體、CAR-T和雙特異性抗體。自1997年全球首款抗CD20單抗——羅氏的利妥昔單抗獲批以來(lái),全球已經(jīng)批準(zhǔn)多款抗CD20單抗。相關(guān)數(shù)據(jù)統(tǒng)計(jì)顯示,目前有高達(dá)90多種針對(duì)CD20的生物制劑處于臨床開(kāi)發(fā)階段。

CUSABIO-哺乳包膜VLPs平臺(tái)成功開(kāi)發(fā)MS4A1全長(zhǎng)膜蛋白(4次跨膜),展示了完整生物活性。

熱銷(xiāo)產(chǎn)品

Recombinant Human B-lymphocyte antigen CD20(MS4A1)-VLPs (Active) CSB-MP015007HU

驗(yàn)證數(shù)據(jù)

High Specificity Validated by Western blot (WB)

CSB-MP015007HU is detected by Mouse anti-6*His monoclonal antibody.

Excellent Bioactivity Validated by Functional ELISA

Measured by its binding ability in a functional ELISA. Immobilized human CD20 at 2 μg/ml can bind Anti-CD20 recombinant antibody (CSB-RA015007A1HU), the EC50 is 3.243-7.085 ng/mL.

Correct Structure Validated
by TEM

The presence of VLP-like structures was confirmed by TEM.

MS4A1 Recombinant Monoclonal Antibody CSB-RA015007MA4HU

驗(yàn)證數(shù)據(jù)

WB
CSB-RA015007MA4HU WB

IHC image of CSB-RA015007MA4HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB

IHC
CSB-RA015007MA4HU IF

Immunofluorescence staining of Hela cell with CSB-RA015007MA4HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

IF
CSB-RA015007MA4HU IF

Immunofluorescence staining of MCF7 cell with CSB-RA015007MA4HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L)

MS4A1 Antibodies

MS4A1 for Mus musculus (Mouse)

MS4A1 for Homo sapiens (Human)

MS4A1 for H, M, R

MS4A1 Proteins

MS4A1 Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

MS4A1 Proteins for Mus musculus (Mouse)

MS4A1 Proteins for Homo sapiens (Human)

MS4A1 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)

MS4A1 ELISA Kit

MS4A1 ELISA Kit for Homo sapiens (Human)



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