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HNF4A

HNF4A,即肝細胞核因子4α,別名NR2A1、TCF14、MODY1。HNF4A是一種核受體超家族成員,屬于配體依賴性轉錄因子,主要分布在肝臟中,在肝臟的發育和肝細胞分化成熟過程中起重要調控作用。HNF4A在轉錄水平上調控肝臟特異基因的表達,促進肝細胞的發育和分化,參與肝細胞極性的建立和維持,增強肝臟的合成、代謝及解毒功能。HNF4A通過抑制肝星狀細胞的活化、逆轉上皮間質細胞轉化、抑制肝癌細胞增殖、侵襲和轉移等機制,參與肝纖維化、肝硬化和肝細胞癌等疾病的發生和變化過程。HNF4A的表達異常與多種肝臟疾病相關,包括肝纖維化、肝硬化和肝癌。

熱銷產品

Recombinant Human Hepatocyte nuclear factor 4-alpha (HNF4A) (CSB-EP010594HU)

驗證數據

CSB-EP010594HU

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP010594HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) HNF4A.

CSB-EP010594HU

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP010594HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) HNF4A.

HNF4A Recombinant Monoclonal Antibody (CSB-RA571090A0HU)

驗證數據

CSB-RA571090A0HU

Western Blot
Positive WB detected in: HL-60 whole cell lysate, Rat Liver tissue lysate,
All lanes: HNF-4-alpha antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

CSB-RA571090A0HU

IHC image of CSB-RA571090A0HU diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.75% DAB.

CSB-RA571090A0HU

Immunofluorescence staining of MCF-7 with CSB-RA571090A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 526-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CSB-RA571090A0HU

Overlay Peak curve showing HepG2 cells stained with CSB-RA571090A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

HNF4A Antibodies

HNF4A for Homo sapiens (Human)

HNF4A Proteins

HNF4A Proteins for Rattus norvegicus (Rat)

HNF4A Proteins for Homo sapiens (Human)

HNF4A Proteins for Mus musculus (Mouse)

HNF4A Proteins for Xenopus laevis (African clawed frog)



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