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Your Good Partner in Biology Research

BRAP

BRAP,全稱為BRCA1相關(guān)蛋白(BRCA1-associated protein),也被稱為BAP2、IMP或RNF52。該蛋白屬于RING指蛋白家族,具有一個保守的RING結(jié)構(gòu)域,參與蛋白質(zhì)間的相互作用,尤其是在泛素化和DNA損傷修復(fù)過程中。BRAP蛋白主要在細胞核內(nèi)表達,與BRCA1等蛋白結(jié)合,影響腫瘤抑制功能,在維持基因組穩(wěn)定性中起關(guān)鍵作用。
BRAP蛋白的異常表達與多種疾病相關(guān),如心血管疾病和自身免疫性疾病。在腫瘤領(lǐng)域,BRAP蛋白的高表達與基因組不穩(wěn)定性增加有關(guān),從而提升癌癥風險,如乳腺癌、卵巢癌等。基于BRAP蛋白的特性,研究人員正在探索其作為潛在治療靶點的可能性。目前,已有研究顯示BRAP蛋白在食管癌的侵襲轉(zhuǎn)移中發(fā)揮重要作用,其高表達能夠促進食管癌細胞的遷移侵襲能力,并降低患者的生存時間。此外,BRAP蛋白也被認為是新冠肺炎抗炎藥物研發(fā)的潛在靶點之一。

熱銷產(chǎn)品

Recombinant Human BRCA1-associated protein (BRAP),Partial (CSB-EP773793HU(M))

驗證數(shù)據(jù)

CSB-EP773793HU(M)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

BRAP Antibody (CSB-PA773793LA01HU)

驗證數(shù)據(jù)

CSB-PA773793LA01HU

Western Blot
Positive WB detected in: Mouse Brain tissue lysate, Mouse Heart tissue lysate, Rat Heart tissue lysate,
All lanes: BRAP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa

CSB-PA773793LA01HU

Western Blot
Positive WB detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate
All lanes: BRAP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa

CSB-PA773793LA01HU

IHC image of CSB-PA773793LA01HU diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.

CSB-PA773793LA01HU

Immunofluorescence staining of Hela cell with CSB-PA773793LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CSB-PA773793LA01HU

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

BRAP Antibodies

BRAP for Homo sapiens (Human)

BRAP Proteins

BRAP Proteins for Homo sapiens (Human)

BRAP Proteins for Mus musculus (Mouse)



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