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  4. PPIE Antibody

PPIE Antibody

  • 中文名稱：
    PPIE兔多克隆抗體
  • 貨號(hào)：
    CSB-PA890944LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • IHC image of CSB-PA890944LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA890944LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) PPIE Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    PPIE
  • 別名：
    Ab1-210 antibody; Cyclophilin 33 antibody; Cyclophilin E antibody; Cyclophilin-33 antibody; CYP 33 antibody; CYP33 antibody; EC 5.2.1.8 antibody; Peptidyl prolyl cis trans isomerase E antibody; Peptidyl-prolyl cis-trans isomerase E antibody; Peptidylprolyl isomerase E (cyclophilin E) antibody; Peptidylprolyl isomerase E antibody; PPIase E antibody; PPIE antibody; PPIE_HUMAN antibody; Rotamase E antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human Peptidyl-prolyl cis-trans isomerase E protein (86-141AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁(yè)面中的產(chǎn)品，PPIE Antibody (CSB-PA890944LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于PPIE Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號(hào) 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA890944LB01HU PPIE Antibody, HRP conjugated ELISA
    FITC CSB-PA890944LC01HU PPIE Antibody, FITC conjugated
    Biotin CSB-PA890944LD01HU PPIE Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:200-1:500
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.
  • 基因功能參考文獻(xiàn)：
    1. CypE is a host restriction factor that inhibits the functions of nucleoprotein, as well as viral replication and transcription, by impairing the formation of the viral ribonucleoprotein complex. PMID: 21887220
    2. binding of H3K4me3 to PHD3 domain of MLL and binding of the CYP33 RRM domain to PHD3 are mutually inhibitory, implying that PHD3 is a molecular switch for the transition between activation and repression of target genes PMID: 20677832
    3. PPIase domain of CyP33 regulates the conformation of MLL1 through proline isomerization within the PHD3-Bromo linker, thereby disrupting the PHD3-Bromo interface and facilitating binding of the MLL1-PHD3 domain to the CyP33-RRM domain. PMID: 20541251
    4. Data provide insight into the multiple functions of Cyp33 RRM and suggest a Cyp33-dependent mechanism for regulating the transcriptional activity of MLL. PMID: 20460131
    5. The results show that hCyP33 binds specifically to mRNA, namely poly(A)(+)RNA, and that binding stimulates the PPIase activity of hCyP33. PMID: 18258190

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Cyclophilin-type PPIase family, PPIase E subfamily
  • 組織特異性：
    Found in all the examined tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 9258

    OMIM: 602435

    KEGG: hsa:10450

    UniGene: Hs.524690



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