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  4. Human Apolipoprotein F(APOF) ELISA kit

Human Apolipoprotein F(APOF) ELISA kit

  • 中文名稱：
    人載脂蛋白F(APOF)酶聯免疫試劑盒
  • 貨號：
    CSB-EL001938HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人載脂蛋白F(APOF)酶聯免疫試劑盒（CSB-EL001938HU）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOF含量。APOF（載脂蛋白F）是一種酸性唾液酸蛋白，存在于高密度和低密度脂蛋白上。研究表明，ApoF在動脈粥樣硬化形成中可能發揮重要作用，其作用機制可能與血漿膽固醇水平不直接相關。近期研究顯示，ApoF通過調節膽固醇代謝和胰島β細胞功能，影響胰島素分泌，從而在糖尿病等疾病中發揮作用。試劑盒檢測范圍為9.38 ng/mL-600 ng/mL，支持科研人員開展脂代謝機制、心血管疾病模型構建等基礎研究，尤其適用于評估動物實驗模型中肝臟、脂肪組織等部位的APOF表達水平，或探索其在脂質運輸、炎癥反應等分子通路中的功能；可滿足細胞實驗、疾病模型構建及相關生物標志物篩選等科研場景需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Apo-F ELISA Kit; Apof ELISA Kit; APOF_HUMAN ELISA Kit; Apolipoprotein F ELISA Kit; DKFZp781G18150 ELISA Kit; Lipid transfer inhibitor protein ELISA Kit; LTIP ELISA Kit; MGC22520 ELISA Kit
  • 縮寫：
    APOF
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    9.38 ng/mL-600 ng/mL
  • 靈敏度：
    2.34 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human APOF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:200 Average % 92
    Range % 86-96
    1:400 Average % 95
    Range % 91-99
    1:800 Average % 88
    Range % 84-94
    1:1600 Average % 87
    Range % 84-93
  • 回收率：
    The recovery of human APOF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 90-98
    EDTA plasma (n=4) 95 90-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average
    600 0.088 0.090 0.089
    300 0.113 0.119 0.116
    150 0.161 0.172 0.167
    75 0.243 0.254 0.249
    37.5 0.388 0.395 0.392
    18.75 0.680 0.698 0.689
    9.38 0.941 1.001 0.971
    0 1.975 2.075 2.025
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
  • 基因功能參考文獻：
    1. Direct interactions between C/EBPalpha and ETS-1 were important for high liver-specific expression of ApoF. PMID: 25726912
    2. this study has identified apoF as likely another target gene of FXR. PMID: 24211198
    3. LTIP translocation is dependent on LDL lipid composition PMID: 21937674
    4. LTIP tailors CETP-mediated remodeling of HDL3 and HDL2 particles in subclass-specific ways, strongly implicating it as a regulator of HDL metabolism PMID: 12907677
    5. we have confirmed the negative association between plasma triglyceride levels and Apolipoprotein F previously suggested by a small data set, but now we demonstrate that this effect is seen only in males. PMID: 17901467
    6. lipoprotein composition may influence the status of lipid transfer inhibitor protein activity PMID: 18369235

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein F family
  • 組織特異性：
    Expressed by the liver and secreted in plasma.
  • 數據庫鏈接：

    HGNC: 615

    OMIM: 107760

    KEGG: hsa:319

    STRING: 9606.ENSP00000381250

    UniGene: Hs.534302



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