在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli Dihydrofolate reductase (folA)

In Stock
  • 中文名稱:
    大腸桿菌folA重組蛋白
  • 貨號(hào):
    CSB-EP006847ENV
  • 規(guī)格:
    ¥1836
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    folA
  • Uniprot No.:
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長(zhǎng)度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    18.8 kDa
  • 表達(dá)區(qū)域:
    1-159aa
  • 氨基酸序列
    MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
  • 基因功能參考文獻(xiàn):
    1. NADP+ not only binds to the native form but also a partially unfolded form of dihydrofolate reductase. PMID: 25367157
    2. Quantum mechanics/molecular dynamics simulations reveal that the M20 loop conformational dynamics of dihydrofolate reductase (DHFR) is severely restricted at the transition state of the hydride transfer as a result of the M42W/G121V double mutation. PMID: 23297871
    3. Side-chain conformational heterogeneity of intermediates in the Escherichia coli dihydrofolate reductase catalytic cycle PMID: 23614825
    4. The data presented here provide a glimpse into the evolutionary trajectory of functional DHFR through its protein sequence space that lead to the diverged binding and catalytic properties of the E. coli and human enzymes. PMID: 23733948
    5. Present a general kinetic framework that can be used to study conformation changes, apply this framework to E. coli DHFR and find the conformational change occurs predominantly prior to unbinding. PMID: 22641560
    6. Protein interface remodeling in a chemically induced protein dimer. PMID: 22733548
    7. Dihydrofolate reductase is bound to endogenous tetrahydrofolate PMID: 22024482
    8. [review] A general mechanism is presented for folA catalysis that includes multiple intermediates and a complex, multidimensional standard free energy surface. PMID: 22029278
    9. Only a single peptide from DHFR is found to be substantially more flexible than the Bacillus stearothermophilus-DHFR at 25 degrees C in a region located within the protein interior at the intersection of the cofactor and substrate-binding sites. PMID: 21859100
    10. Taken together with previous studies in the millisecond time range, a hierarchical assembly of DHFR--in which each subdomain independently folds, subsequently docks, and then anneals into the native conformation after an initial global collapse--emerges. PMID: 21554889
    11. Thermodynamics and solvent effects on substrate and cofactor binding in Escherichia coli chromosomal dihydrofolate reductase PMID: 21462996
    12. mutant DHFR that abrogates millisecond-time-scale fluctuation in active site without perturbing structural and electrostatic preorganization; found link between conformational fluctuations on millisecond time scale and chemical step of enzymatic reaction PMID: 21474759
    13. Fcused on residues 52, 67, 121, and 145 in the four distinct loops of DHFR. All the single-residue deletion mutants showed marked reduction in stability, except for Delta52 in an alphaC-betaC loop. PMID: 20045086
    14. resulting triple mutants, DM-N18C, DM-R52C, DM-D87C and DM-D132C dihydrofolate reductase, were alkylated with glucose, N-acetylglucosamine, lactose and maltotriose iodoacetamides. PMID: 20412060
    15. Data show that the M42W mutation alters the dynamics of DHFR and are consistent with theoretical analysis that suggests this mutation disrupts motion that promotes catalysis. PMID: 20073522
    16. results suggest that dynamics in dihydrofolate reductase are exquisitely "tuned" for every intermediate in the catalytic cycle; structural fluctuations efficiently channel the enzyme through functionally relevant conformational space. PMID: 20080605
    17. These results suggest that through electrostatic interactions Arg44 plays a functional role in retaining the cofactor binding affinity at the cost of the Escherichia coli dihydrofolate reductase stability. PMID: 20043879
    18. Lys-32 residues have a role in the ionic interaction in R67 dihydrofolate reductase PMID: 15333636
    19. the hydroxyl group of Tyr-69 of DFHR is important for interactions with NADPH, whereas both the hydroxyl group and hydrophobic ring atoms of the Tyr-69 residues are necessary for proper interactions with dihydrofolate PMID: 15333637
    20. structural and functional alterations induced by peroxynitrite may play a direct role in compromising DHFR function in multiple pathological conditions PMID: 15639221
    21. biophysical analysis of immobilized and native Escherichia coli dihydrofolate reductase PMID: 16258053
    22. Results show that mutant dihydrofolate reductase has reduced catalytic activity. PMID: 16363797
    23. characterization of higher energy conformational substates of dihydrofolate reductase using using nuclear magnetic resonance relaxation dispersion PMID: 16973882
    24. DHFR structure from neutron diffraction studies provides insights into dynamics, active-site protonation states, and solvation pattern of the E. coli enzyme. PMID: 17130456
    25. The folding trajectory of this alpha/beta-type protein (DHFR) is located between those of alpha-helical and beta-sheet proteins, suggesting that native structure determines the folding landscape. PMID: 17331539
    26. Several mutations were found to grant resistance to trimethoprim, both by reducing the binding affinity of the enzyme for the drug, and by increasing the activity of the enzyme. PMID: 17451440

    顯示更多

    收起更多

  • 蛋白家族:
    Dihydrofolate reductase family
  • 數(shù)據(jù)庫鏈接:


主站蜘蛛池模板: 在线天堂资源www在线中文| 性色av一区二区三区无码| 久久亚洲a片com人成| 2021年国产精品专区丝袜| 亚洲国产av无码一区二区三区| 最近最新中文字幕高清免费| 女人被黑人狂躁c到高潮小说| 黑人巨茎大战欧美白妇| 亚洲伊人色综合www962| 久久精品亚洲中文无东京热| av制服丝袜白丝国产网站 | 鲁大师在线视频播放免费观看 | 国产亚洲精品久久久999蜜臀| 国产边打电话边被躁视频| 亚洲国产日韩精品一区二区三区| 少妇的肉体k8经典| av无码久久久精品免费| 欧美自拍另类欧美综合图片区 | 午夜天堂精品久久久久| 精品国产杨幂在线观看| 99精品国产高清一区二区麻豆| 视频分类 国内精品| 成人免费的视频| 青青草国产免费久久久| 欧美成人www免费全部网站| 欧美牲交a欧美牲交aⅴ免费真 | 国产免费啪嗒啪嗒视频看看| 亚洲国产品综合人成综合网站| 国产精品久久久久久久久久久免费看| 无码无遮挡又大又爽又黄的视频| 专干熟肥老妇人视频在线看| 日本免费一区二区三区四区五区| 亚洲一卡二卡三卡四卡| 欧美精品欧美人与动人物牲交| 欧美日韩亚洲国产综合乱| 丰满少妇熟乱xxxxx视频| 中文字幕日韩精品无码内射| 少妇精品偷拍高潮少妇小说| 国产乱妇无码大片在线观看| 少妇av一区二区三区无码| 久久99精品久久久久久齐齐|