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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nematostella vectensis UPF0587 protein v1g245604 (v1g245604) CSB-YP417273NGO
CSB-EP417273NGO
CSB-BP417273NGO
CSB-MP417273NGO
CSB-EP417273NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP417274YAN
CSB-EP417274YAN
CSB-BP417274YAN
CSB-MP417274YAN
CSB-EP417274YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP417275YAN1
CSB-EP417275YAN1
CSB-BP417275YAN1
CSB-MP417275YAN1
CSB-EP417275YAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP417276YAN
CSB-EP417276YAN
CSB-BP417276YAN
CSB-MP417276YAN
CSB-EP417276YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 50S ribosomal protein L32 (rpmF) CSB-YP417277YAN
CSB-EP417277YAN
CSB-BP417277YAN
CSB-MP417277YAN
CSB-EP417277YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b High frequency lysogenization protein HflD homolog (hflD) CSB-YP417278YAN
CSB-EP417278YAN
CSB-BP417278YAN
CSB-MP417278YAN
CSB-EP417278YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Protease HtpX (htpX), partial CSB-YP417279YAN1
CSB-EP417279YAN1
CSB-BP417279YAN1
CSB-MP417279YAN1
CSB-EP417279YAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP417280YAN
CSB-EP417280YAN
CSB-BP417280YAN
CSB-MP417280YAN
CSB-EP417280YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0061 protein YpsIP31758_1734 (YpsIP31758_1734) CSB-YP417281YAN
CSB-EP417281YAN
CSB-BP417281YAN
CSB-MP417281YAN
CSB-EP417281YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Ribonuclease T (rnt) CSB-YP417282YAN
CSB-EP417282YAN
CSB-BP417282YAN
CSB-MP417282YAN
CSB-EP417282YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Electron transport complex protein RnfD (rnfD), partial CSB-YP417283YAN
CSB-EP417283YAN
CSB-BP417283YAN
CSB-MP417283YAN
CSB-EP417283YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0259 membrane protein YpsIP31758_1942 (YpsIP31758_1942), partial CSB-YP417284YAN1
CSB-EP417284YAN1
CSB-BP417284YAN1
CSB-MP417284YAN1
CSB-EP417284YAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0229 protein YpsIP31758_1992 (YpsIP31758_1992) CSB-YP417285YAN
CSB-EP417285YAN
CSB-BP417285YAN
CSB-MP417285YAN
CSB-EP417285YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0260 protein YpsIP31758_2006 (YpsIP31758_2006) CSB-YP417286YAN
CSB-EP417286YAN
CSB-BP417286YAN
CSB-MP417286YAN
CSB-EP417286YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b tRNA (cmo5U34)-methyltransferase CSB-YP417287YAN
CSB-EP417287YAN
CSB-BP417287YAN
CSB-MP417287YAN
CSB-EP417287YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP417288YAN
CSB-EP417288YAN
CSB-BP417288YAN
CSB-MP417288YAN
CSB-EP417288YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Flagellar protein FliT (fliT) CSB-YP417289YAN
CSB-EP417289YAN
CSB-BP417289YAN
CSB-MP417289YAN
CSB-EP417289YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP417290YAN
CSB-EP417290YAN
CSB-BP417290YAN
CSB-MP417290YAN
CSB-EP417290YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0265 protein YpsIP31758_2417 (YpsIP31758_2417) CSB-YP417291YAN
CSB-EP417291YAN
CSB-BP417291YAN
CSB-MP417291YAN
CSB-EP417291YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP417292YAN
CSB-EP417292YAN
CSB-BP417292YAN
CSB-MP417292YAN
CSB-EP417292YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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