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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. holarctica 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP415813FDV
CSB-EP415813FDV
CSB-BP415813FDV
CSB-MP415813FDV
CSB-EP415813FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Isoleucine--tRNA ligase (ileS), partial CSB-YP415814FDV
CSB-EP415814FDV
CSB-BP415814FDV
CSB-MP415814FDV
CSB-EP415814FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Glycogen synthase (glgA) CSB-YP415815FDV
CSB-EP415815FDV
CSB-BP415815FDV
CSB-MP415815FDV
CSB-EP415815FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP415816FDV
CSB-EP415816FDV
CSB-BP415816FDV
CSB-MP415816FDV
CSB-EP415816FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP415817FDV
CSB-EP415817FDV
CSB-BP415817FDV
CSB-MP415817FDV
CSB-EP415817FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L32 (rpmF) CSB-YP415818FDV
CSB-EP415818FDV
CSB-BP415818FDV
CSB-MP415818FDV
CSB-EP415818FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Chaperone protein DnaK (dnaK), partial CSB-YP415819FDV
CSB-EP415819FDV
CSB-BP415819FDV
CSB-MP415819FDV
CSB-EP415819FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Biotin synthase (bioB) CSB-YP415820FDV
CSB-EP415820FDV
CSB-BP415820FDV
CSB-MP415820FDV
CSB-EP415820FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Alanine racemase (alr) CSB-YP415821FDV
CSB-EP415821FDV
CSB-BP415821FDV
CSB-MP415821FDV
CSB-EP415821FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica UPF0133 protein FTA_1499 (FTA_1499) CSB-YP415822FDV
CSB-EP415822FDV
CSB-BP415822FDV
CSB-MP415822FDV
CSB-EP415822FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Catalase-peroxidase (katG), partial CSB-YP415823FDV
CSB-EP415823FDV
CSB-BP415823FDV
CSB-MP415823FDV
CSB-EP415823FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP415824FDV
CSB-EP415824FDV
CSB-BP415824FDV
CSB-MP415824FDV
CSB-EP415824FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP415825FDV
CSB-EP415825FDV
CSB-BP415825FDV
CSB-MP415825FDV
CSB-EP415825FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP415826FDV
CSB-EP415826FDV
CSB-BP415826FDV
CSB-MP415826FDV
CSB-EP415826FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 30S ribosomal protein S16 (rpsP) CSB-YP415827FDV
CSB-EP415827FDV
CSB-BP415827FDV
CSB-MP415827FDV
CSB-EP415827FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Phosphoglucosamine mutase (glmM) CSB-YP415828FDV
CSB-EP415828FDV
CSB-BP415828FDV
CSB-MP415828FDV
CSB-EP415828FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Histidine--tRNA ligase (hisS) CSB-YP415829FDV
CSB-EP415829FDV
CSB-BP415829FDV
CSB-MP415829FDV
CSB-EP415829FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP415830FDV
CSB-EP415830FDV
CSB-BP415830FDV
CSB-MP415830FDV
CSB-EP415830FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP415831FDV
CSB-EP415831FDV
CSB-BP415831FDV
CSB-MP415831FDV
CSB-EP415831FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Demethylmenaquinone methyltransferase (ubiE) CSB-YP415832RIP
CSB-EP415832RIP
CSB-BP415832RIP
CSB-MP415832RIP
CSB-EP415832RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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