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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Rhomboid protease glpG (glpG), partial CSB-YP374327YAK1
CSB-EP374327YAK1
CSB-BP374327YAK1
CSB-MP374327YAK1
CSB-EP374327YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Glycogen synthase (glgA) CSB-YP374328YAK
CSB-EP374328YAK
CSB-BP374328YAK
CSB-MP374328YAK
CSB-EP374328YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP374329YAK
CSB-EP374329YAK
CSB-BP374329YAK
CSB-MP374329YAK
CSB-EP374329YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0194 membrane protein YE2891 (YE2891) CSB-YP374330YAK
CSB-EP374330YAK
CSB-BP374330YAK
CSB-MP374330YAK
CSB-EP374330YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Flagellar protein FliT (fliT) CSB-YP374331YAK
CSB-EP374331YAK
CSB-BP374331YAK
CSB-MP374331YAK
CSB-EP374331YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Flagellar hook-basal body complex protein FliE (fliE) CSB-YP374332YAK
CSB-EP374332YAK
CSB-BP374332YAK
CSB-MP374332YAK
CSB-EP374332YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Xylose isomerase (xylA) CSB-YP374333YAK
CSB-EP374333YAK
CSB-BP374333YAK
CSB-MP374333YAK
CSB-EP374333YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Flagella synthesis protein flgN (flgN) CSB-YP374334YAK
CSB-EP374334YAK
CSB-BP374334YAK
CSB-MP374334YAK
CSB-EP374334YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP374335YAK
CSB-EP374335YAK
CSB-BP374335YAK
CSB-MP374335YAK
CSB-EP374335YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Chromosomal replication initiator protein DnaA (dnaA) CSB-YP374336YAK
CSB-EP374336YAK
CSB-BP374336YAK
CSB-MP374336YAK
CSB-EP374336YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B ATP synthase subunit c (atpE), partial CSB-YP374337YAK1
CSB-EP374337YAK1
CSB-BP374337YAK1
CSB-MP374337YAK1
CSB-EP374337YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Pesticin receptor (fyuA), partial CSB-YP374338YAK
CSB-EP374338YAK
CSB-BP374338YAK
CSB-MP374338YAK
CSB-EP374338YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP374339YAK
CSB-EP374339YAK
CSB-BP374339YAK
CSB-MP374339YAK
CSB-EP374339YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Probable propionate kinase (pduW) CSB-YP374340YAK
CSB-EP374340YAK
CSB-BP374340YAK
CSB-MP374340YAK
CSB-EP374340YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0265 protein YE2755 (YE2755) CSB-YP374341YAK
CSB-EP374341YAK
CSB-BP374341YAK
CSB-MP374341YAK
CSB-EP374341YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Histidinol-phosphate aminotransferase (hisC) CSB-YP374342YAK
CSB-EP374342YAK
CSB-BP374342YAK
CSB-MP374342YAK
CSB-EP374342YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Uridine kinase (udk) CSB-YP374343YAK
CSB-EP374343YAK
CSB-BP374343YAK
CSB-MP374343YAK
CSB-EP374343YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP374344YAK
CSB-EP374344YAK
CSB-BP374344YAK
CSB-MP374344YAK
CSB-EP374344YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Chaperone protein sycT (sycT) CSB-YP374345YAK
CSB-EP374345YAK
CSB-BP374345YAK
CSB-MP374345YAK
CSB-EP374345YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Chaperone protein yscY (yscY) CSB-YP374346YAK
CSB-EP374346YAK
CSB-BP374346YAK
CSB-MP374346YAK
CSB-EP374346YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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