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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neisseria meningitidis serogroup A / serotype 4A tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP884978NGF
CSB-EP884978NGF
CSB-BP884978NGF
CSB-MP884978NGF
CSB-EP884978NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Ribose-5-phosphate isomerase A (rpiA) CSB-YP884979NGF
CSB-EP884979NGF
CSB-BP884979NGF
CSB-MP884979NGF
CSB-EP884979NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Tryptophan--tRNA ligase (trpS) CSB-YP884980NGF
CSB-EP884980NGF
CSB-BP884980NGF
CSB-MP884980NGF
CSB-EP884980NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP884981NGF
CSB-EP884981NGF
CSB-BP884981NGF
CSB-MP884981NGF
CSB-EP884981NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP884982NGF
CSB-EP884982NGF
CSB-BP884982NGF
CSB-MP884982NGF
CSB-EP884982NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Primosomal replication protein n (priB) CSB-YP884983NGF
CSB-EP884983NGF
CSB-BP884983NGF
CSB-MP884983NGF
CSB-EP884983NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Aspartate 1-decarboxylase (panD) CSB-YP884984NGF
CSB-EP884984NGF
CSB-BP884984NGF
CSB-MP884984NGF
CSB-EP884984NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP884985NGF
CSB-EP884985NGF
CSB-BP884985NGF
CSB-MP884985NGF
CSB-EP884985NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A [Protein-PII] uridylyltransferase (glnD), partial CSB-YP884986NGF
CSB-EP884986NGF
CSB-BP884986NGF
CSB-MP884986NGF
CSB-EP884986NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Putative ankyrin repeat protein NMA1343 (NMA1343) CSB-YP884987NGF
CSB-EP884987NGF
CSB-BP884987NGF
CSB-MP884987NGF
CSB-EP884987NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Gamma-glutamyl phosphate reductase (proA) CSB-YP884988NGF
CSB-EP884988NGF
CSB-BP884988NGF
CSB-MP884988NGF
CSB-EP884988NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A 50S ribosomal protein L31 type B (rpmE2) CSB-YP884989NGF
CSB-EP884989NGF
CSB-BP884989NGF
CSB-MP884989NGF
CSB-EP884989NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A UPF0246 protein NMA1114 (NMA1114) CSB-YP884990NGF
CSB-EP884990NGF
CSB-BP884990NGF
CSB-MP884990NGF
CSB-EP884990NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Recombination-associated protein RdgC (rdgC) CSB-YP884991NGF
CSB-EP884991NGF
CSB-BP884991NGF
CSB-MP884991NGF
CSB-EP884991NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A GTP cyclohydrolase folE2 (folE2) CSB-YP884992NGF
CSB-EP884992NGF
CSB-BP884992NGF
CSB-MP884992NGF
CSB-EP884992NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A UPF0042 nucleotide-binding protein NMA0948 (NMA0948) CSB-YP884993NGF
CSB-EP884993NGF
CSB-BP884993NGF
CSB-MP884993NGF
CSB-EP884993NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Threonine--tRNA ligase (thrS), partial CSB-YP884994NGF
CSB-EP884994NGF
CSB-BP884994NGF
CSB-MP884994NGF
CSB-EP884994NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Ribonuclease 3 (rnc) CSB-YP884995NGF
CSB-EP884995NGF
CSB-BP884995NGF
CSB-MP884995NGF
CSB-EP884995NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Thymidylate kinase (tmk) CSB-YP884996NGF
CSB-EP884996NGF
CSB-BP884996NGF
CSB-MP884996NGF
CSB-EP884996NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Peptide chain release factor 3 (prfC), partial CSB-YP884997NGF
CSB-EP884997NGF
CSB-BP884997NGF
CSB-MP884997NGF
CSB-EP884997NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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