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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xanthomonas campestris pv. campestris UPF0234 protein XCC3632 (XCC3632) CSB-YP851051XAY
CSB-EP851051XAY
CSB-BP851051XAY
CSB-MP851051XAY
CSB-EP851051XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Periplasmic trehalase (treA), partial CSB-YP851052XAY
CSB-EP851052XAY
CSB-BP851052XAY
CSB-MP851052XAY
CSB-EP851052XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris UPF0250 protein XCC3453 (XCC3453) CSB-YP851053XAY
CSB-EP851053XAY
CSB-BP851053XAY
CSB-MP851053XAY
CSB-EP851053XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Phosphomethylpyrimidine synthase (thiC), partial CSB-YP851054XAY
CSB-EP851054XAY
CSB-BP851054XAY
CSB-MP851054XAY
CSB-EP851054XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Non-canonical purine NTP pyrophosphatase (XCC3253) CSB-YP851055XAY
CSB-EP851055XAY
CSB-BP851055XAY
CSB-MP851055XAY
CSB-EP851055XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Probable fructose-bisphosphate aldolase class 1 (XCC3185) CSB-YP851056XAY
CSB-EP851056XAY
CSB-BP851056XAY
CSB-MP851056XAY
CSB-EP851056XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris UPF0213 protein XCC3072 (XCC3072) CSB-YP851057XAY
CSB-EP851057XAY
CSB-BP851057XAY
CSB-MP851057XAY
CSB-EP851057XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Probable potassium transport system protein kup (kup), partial CSB-YP851058XAY
CSB-EP851058XAY
CSB-BP851058XAY
CSB-MP851058XAY
CSB-EP851058XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP851059XAY1
CSB-EP851059XAY1
CSB-BP851059XAY1
CSB-MP851059XAY1
CSB-EP851059XAY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP851060XAY
CSB-EP851060XAY
CSB-BP851060XAY
CSB-MP851060XAY
CSB-EP851060XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP851061XAY
CSB-EP851061XAY
CSB-BP851061XAY
CSB-MP851061XAY
CSB-EP851061XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Endonuclease V (nfi) CSB-YP851062XAY
CSB-EP851062XAY
CSB-BP851062XAY
CSB-MP851062XAY
CSB-EP851062XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Maf-like protein XCC2610 (XCC2610) CSB-YP851063XAY
CSB-EP851063XAY
CSB-BP851063XAY
CSB-MP851063XAY
CSB-EP851063XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L3 glutamine methyltransferase (prmB) CSB-YP851064XAY
CSB-EP851064XAY
CSB-BP851064XAY
CSB-MP851064XAY
CSB-EP851064XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Phosphoglucosamine mutase (glmM) CSB-YP851065XAY
CSB-EP851065XAY
CSB-BP851065XAY
CSB-MP851065XAY
CSB-EP851065XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP851066XAY
CSB-EP851066XAY
CSB-BP851066XAY
CSB-MP851066XAY
CSB-EP851066XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Translation initiation factor IF-3 (infC) CSB-YP851067XAY
CSB-EP851067XAY
CSB-BP851067XAY
CSB-MP851067XAY
CSB-EP851067XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP851068XAY
CSB-EP851068XAY
CSB-BP851068XAY
CSB-MP851068XAY
CSB-EP851068XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP851069XAY
CSB-EP851069XAY
CSB-BP851069XAY
CSB-MP851069XAY
CSB-EP851069XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Cysteine--tRNA ligase (cysS) CSB-YP851070XAY
CSB-EP851070XAY
CSB-BP851070XAY
CSB-MP851070XAY
CSB-EP851070XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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