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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacteroides thetaiotaomicron NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP804246BDU
CSB-EP804246BDU
CSB-BP804246BDU
CSB-MP804246BDU
CSB-EP804246BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Methionyl-tRNA formyltransferase (fmt) CSB-YP804247BDU
CSB-EP804247BDU
CSB-BP804247BDU
CSB-MP804247BDU
CSB-EP804247BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Malate dehydrogenase (mdh) CSB-YP804248BDU
CSB-EP804248BDU
CSB-BP804248BDU
CSB-MP804248BDU
CSB-EP804248BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron UPF0246 protein BT_3869 (BT_3869) CSB-YP804249BDU
CSB-EP804249BDU
CSB-BP804249BDU
CSB-MP804249BDU
CSB-EP804249BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron L-rhamnose isomerase (rhaA) CSB-YP804250BDU
CSB-EP804250BDU
CSB-BP804250BDU
CSB-MP804250BDU
CSB-EP804250BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Putative glycoside hydrolase BT_3595 (BT_3595), partial CSB-YP804252BDU
CSB-EP804252BDU
CSB-BP804252BDU
CSB-MP804252BDU
CSB-EP804252BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron DNA repair protein recO (recO) CSB-YP804253BDU
CSB-EP804253BDU
CSB-BP804253BDU
CSB-MP804253BDU
CSB-EP804253BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Translation initiation factor IF-2 (infB), partial CSB-YP804254BDU
CSB-EP804254BDU
CSB-BP804254BDU
CSB-MP804254BDU
CSB-EP804254BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Probable rRNA maturation factor (BT_3283) CSB-YP804255BDU
CSB-EP804255BDU
CSB-BP804255BDU
CSB-MP804255BDU
CSB-EP804255BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Putative membrane protein insertion efficiency factor (BT_3228) CSB-YP804256BDU
CSB-EP804256BDU
CSB-BP804256BDU
CSB-MP804256BDU
CSB-EP804256BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP804257BDU1
CSB-EP804257BDU1
CSB-BP804257BDU1
CSB-MP804257BDU1
CSB-EP804257BDU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Adenosylhomocysteinase (ahcY) CSB-YP804258BDU
CSB-EP804258BDU
CSB-BP804258BDU
CSB-MP804258BDU
CSB-EP804258BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 30S ribosomal protein S10 (rpsJ) CSB-YP804259BDU
CSB-EP804259BDU
CSB-BP804259BDU
CSB-MP804259BDU
CSB-EP804259BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L16 (rplP) CSB-YP804260BDU
CSB-EP804260BDU
CSB-BP804260BDU
CSB-MP804260BDU
CSB-EP804260BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 30S ribosomal protein S8 (rpsH) CSB-YP804261BDU
CSB-EP804261BDU
CSB-BP804261BDU
CSB-MP804261BDU
CSB-EP804261BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Translation initiation factor IF-1 (infA) CSB-YP804262BDU
CSB-EP804262BDU
CSB-BP804262BDU
CSB-MP804262BDU
CSB-EP804262BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG), partial CSB-YP804263BDU
CSB-EP804263BDU
CSB-BP804263BDU
CSB-MP804263BDU
CSB-EP804263BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Uridylate kinase (pyrH) CSB-YP804264BDU
CSB-EP804264BDU
CSB-BP804264BDU
CSB-MP804264BDU
CSB-EP804264BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Dihydroxy-acid dehydratase (ilvD), partial CSB-YP804265BDU
CSB-EP804265BDU
CSB-BP804265BDU
CSB-MP804265BDU
CSB-EP804265BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Ribosome maturation factor RimM (rimM) CSB-YP804266BDU
CSB-EP804266BDU
CSB-BP804266BDU
CSB-MP804266BDU
CSB-EP804266BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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