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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L27 (rpmA) CSB-YP764163BAAE
CSB-EP764163BAAE
CSB-BP764163BAAE
CSB-MP764163BAAE
CSB-EP764163BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP764164BAAE
CSB-EP764164BAAE
CSB-BP764164BAAE
CSB-MP764164BAAE
CSB-EP764164BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0297 protein BT9727_4120 (BT9727_4120) CSB-YP764165BAAE
CSB-EP764165BAAE
CSB-BP764165BAAE
CSB-MP764165BAAE
CSB-EP764165BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP764166BAAE
CSB-EP764166BAAE
CSB-BP764166BAAE
CSB-MP764166BAAE
CSB-EP764166BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Elongation factor 4 (lepA), partial CSB-YP764167BAAE
CSB-EP764167BAAE
CSB-BP764167BAAE
CSB-MP764167BAAE
CSB-EP764167BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP764168BAAE
CSB-EP764168BAAE
CSB-BP764168BAAE
CSB-MP764168BAAE
CSB-EP764168BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian N utilization substance protein B homolog (nusB) CSB-YP764169BAAE
CSB-EP764169BAAE
CSB-BP764169BAAE
CSB-MP764169BAAE
CSB-EP764169BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0403 protein BT9727_3899 (BT9727_3899) CSB-YP764170BAAE
CSB-EP764170BAAE
CSB-BP764170BAAE
CSB-MP764170BAAE
CSB-EP764170BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Biotin synthase (bioB) CSB-YP764171BAAE
CSB-EP764171BAAE
CSB-BP764171BAAE
CSB-MP764171BAAE
CSB-EP764171BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Acireductone dioxygenase (mtnD) CSB-YP764172BAAE
CSB-EP764172BAAE
CSB-BP764172BAAE
CSB-MP764172BAAE
CSB-EP764172BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0356 protein BT9727_3718 (BT9727_3718) CSB-YP764173BAAE
CSB-EP764173BAAE
CSB-BP764173BAAE
CSB-MP764173BAAE
CSB-EP764173BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphopantetheine adenylyltransferase (coaD) CSB-YP764174BAAE
CSB-EP764174BAAE
CSB-BP764174BAAE
CSB-MP764174BAAE
CSB-EP764174BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Bifunctional protein pyrR (pyrR) CSB-YP764175BAAE
CSB-EP764175BAAE
CSB-BP764175BAAE
CSB-MP764175BAAE
CSB-EP764175BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit (pyrK) CSB-YP764176BAAE
CSB-EP764176BAAE
CSB-BP764176BAAE
CSB-MP764176BAAE
CSB-EP764176BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Methionyl-tRNA formyltransferase (fmt) CSB-YP764177BAAE
CSB-EP764177BAAE
CSB-BP764177BAAE
CSB-MP764177BAAE
CSB-EP764177BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribonuclease 3 (rnc) CSB-YP764178BAAE
CSB-EP764178BAAE
CSB-BP764178BAAE
CSB-MP764178BAAE
CSB-EP764178BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L19 (rplS) CSB-YP764179BAAE
CSB-EP764179BAAE
CSB-BP764179BAAE
CSB-MP764179BAAE
CSB-EP764179BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S2 (rpsB) CSB-YP764180BAAE
CSB-EP764180BAAE
CSB-BP764180BAAE
CSB-MP764180BAAE
CSB-EP764180BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribosome-recycling factor (frr) CSB-YP764181BAAE
CSB-EP764181BAAE
CSB-BP764181BAAE
CSB-MP764181BAAE
CSB-EP764181BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S15 (rpsO) CSB-YP764182BAAE
CSB-EP764182BAAE
CSB-BP764182BAAE
CSB-MP764182BAAE
CSB-EP764182BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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