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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Gloeobacter violaceus tRNA pseudouridine synthase B (truB) CSB-YP752171GCI
CSB-EP752171GCI
CSB-BP752171GCI
CSB-MP752171GCI
CSB-EP752171GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP752172CKA
CSB-EP752172CKA
CSB-BP752172CKA
CSB-MP752172CKA
CSB-EP752172CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable malate:quinone oxidoreductase (mqo) CSB-YP752173WBQ
CSB-EP752173WBQ
CSB-BP752173WBQ
CSB-MP752173WBQ
CSB-EP752173WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Uronate isomerase (uxaC) CSB-YP752174VCQ
CSB-EP752174VCQ
CSB-BP752174VCQ
CSB-MP752174VCQ
CSB-EP752174VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0502 protein VVA1225 (VVA1225) CSB-YP752175VCQ
CSB-EP752175VCQ
CSB-BP752175VCQ
CSB-MP752175VCQ
CSB-EP752175VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protoheme IX farnesyltransferase (cyoE), partial CSB-YP752176VCQ
CSB-EP752176VCQ
CSB-BP752176VCQ
CSB-MP752176VCQ
CSB-EP752176VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Cobyric acid synthase (cobQ) CSB-YP752177VCQ
CSB-EP752177VCQ
CSB-BP752177VCQ
CSB-MP752177VCQ
CSB-EP752177VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Serine hydroxymethyltransferase 2 (glyA2) CSB-YP752178VCQ
CSB-EP752178VCQ
CSB-BP752178VCQ
CSB-MP752178VCQ
CSB-EP752178VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus GlcNAc-binding protein A (gbpA) CSB-YP752179VCQ
CSB-EP752179VCQ
CSB-BP752179VCQ
CSB-MP752179VCQ
CSB-EP752179VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Hemin import ATP-binding protein HmuV (hmuV) CSB-YP752180VCQ
CSB-EP752180VCQ
CSB-BP752180VCQ
CSB-MP752180VCQ
CSB-EP752180VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Purine nucleoside phosphorylase DeoD-type 2 (deoD2) CSB-YP752181VCQ
CSB-EP752181VCQ
CSB-BP752181VCQ
CSB-MP752181VCQ
CSB-EP752181VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Anaerobic nitric oxide reductase flavorubredoxin (norV) CSB-YP752182VCQ
CSB-EP752182VCQ
CSB-BP752182VCQ
CSB-MP752182VCQ
CSB-EP752182VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus HTH-type transcriptional regulator malT (malT), partial CSB-YP752183VCQ
CSB-EP752183VCQ
CSB-BP752183VCQ
CSB-MP752183VCQ
CSB-EP752183VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP752184VCQ
CSB-EP752184VCQ
CSB-BP752184VCQ
CSB-MP752184VCQ
CSB-EP752184VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP752185VCQ
CSB-EP752185VCQ
CSB-BP752185VCQ
CSB-MP752185VCQ
CSB-EP752185VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Shikimate dehydrogenase (aroE) CSB-YP752186VCQ
CSB-EP752186VCQ
CSB-BP752186VCQ
CSB-MP752186VCQ
CSB-EP752186VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP752187VCQ
CSB-EP752187VCQ
CSB-BP752187VCQ
CSB-MP752187VCQ
CSB-EP752187VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L10 (rplJ) CSB-YP752188VCQ
CSB-EP752188VCQ
CSB-BP752188VCQ
CSB-MP752188VCQ
CSB-EP752188VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP752189VCQ
CSB-EP752189VCQ
CSB-BP752189VCQ
CSB-MP752189VCQ
CSB-EP752189VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP752190VCQ
CSB-EP752190VCQ
CSB-BP752190VCQ
CSB-MP752190VCQ
CSB-EP752190VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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