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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptomyces griseus subsp. griseus 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP542236FOF
CSB-EP542236FOF
CSB-BP542236FOF
CSB-MP542236FOF
CSB-EP542236FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Gamma-glutamyl phosphate reductase (proA) CSB-YP542237FOF
CSB-EP542237FOF
CSB-BP542237FOF
CSB-MP542237FOF
CSB-EP542237FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Ribonuclease HII (rnhB) CSB-YP542238FOF
CSB-EP542238FOF
CSB-BP542238FOF
CSB-MP542238FOF
CSB-EP542238FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Dihydrodipicolinate reductase (dapB) CSB-YP542239FOF
CSB-EP542239FOF
CSB-BP542239FOF
CSB-MP542239FOF
CSB-EP542239FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP542240FOF
CSB-EP542240FOF
CSB-BP542240FOF
CSB-MP542240FOF
CSB-EP542240FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Ribosome-binding factor A (rbfA) CSB-YP542241FOF
CSB-EP542241FOF
CSB-BP542241FOF
CSB-MP542241FOF
CSB-EP542241FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S16 (rpsP) CSB-YP542242FOF
CSB-EP542242FOF
CSB-BP542242FOF
CSB-MP542242FOF
CSB-EP542242FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP542243FOF
CSB-EP542243FOF
CSB-BP542243FOF
CSB-MP542243FOF
CSB-EP542243FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 3-isopropylmalate dehydrogenase (leuB) CSB-YP542244FOF
CSB-EP542244FOF
CSB-BP542244FOF
CSB-MP542244FOF
CSB-EP542244FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP542245FOF
CSB-EP542245FOF
CSB-BP542245FOF
CSB-MP542245FOF
CSB-EP542245FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Acetate kinase (ackA) CSB-YP542246FOF
CSB-EP542246FOF
CSB-BP542246FOF
CSB-MP542246FOF
CSB-EP542246FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP542247FOF
CSB-EP542247FOF
CSB-BP542247FOF
CSB-MP542247FOF
CSB-EP542247FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP542248FOF
CSB-EP542248FOF
CSB-BP542248FOF
CSB-MP542248FOF
CSB-EP542248FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP542249FOF
CSB-EP542249FOF
CSB-BP542249FOF
CSB-MP542249FOF
CSB-EP542249FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Lanthionine-containing peptide sapB (ramS) CSB-YP542250FOF
CSB-EP542250FOF
CSB-BP542250FOF
CSB-MP542250FOF
CSB-EP542250FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP542251FOF
CSB-EP542251FOF
CSB-BP542251FOF
CSB-MP542251FOF
CSB-EP542251FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP542252FOF
CSB-EP542252FOF
CSB-BP542252FOF
CSB-MP542252FOF
CSB-EP542252FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Pup--protein ligase (pafA) CSB-YP542253FOF
CSB-EP542253FOF
CSB-BP542253FOF
CSB-MP542253FOF
CSB-EP542253FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S4 (rpsD) CSB-YP542254FOF
CSB-EP542254FOF
CSB-BP542254FOF
CSB-MP542254FOF
CSB-EP542254FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP542255FOF
CSB-EP542255FOF
CSB-BP542255FOF
CSB-MP542255FOF
CSB-EP542255FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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