重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質(zhì)，包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應用中發(fā)揮著關鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量（驗證的生物活性，較低的內(nèi)毒素水平和高純度）的重組蛋白質(zhì)來支持您的研究，您來對地方了。
經(jīng)過18年的發(fā)展，華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng)，用于表達重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達和純化技術，包括基于桿狀病毒外膜蛋白（OMP）的蛋白質(zhì)表達技術，以及膜蛋白表達技術等。
產(chǎn)品特點
- 超過13,000種重組蛋白覆蓋大多數(shù)基因/靶點；
- 5種表達系統(tǒng)，包括大腸桿菌（E.coli）、酵母、桿狀病毒感染的昆蟲細胞、哺乳動物細胞和體外大腸桿菌。而體外大腸桿菌表達系統(tǒng)是華美生物的獨特表達系統(tǒng)。與傳統(tǒng)蛋白表達系統(tǒng)相比，體外大腸桿菌省略了許多過程，如質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、收集、破碎和離心等，大大提高了工作效率。
- 多種標簽，包括His、GST、Flag、MBP等。
- 廣泛應用，包括抗原、細胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細胞培養(yǎng)-無血清培養(yǎng)基、藥物相關研究、體外酶活性、ELISA標準及其原料、體內(nèi)研究、質(zhì)譜標準、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析（晶體/電子顯微鏡）。
- 多種物種，包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體，請查看華美生物蛋白質(zhì)定制服務。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號 | 規(guī)格 |
|---|---|---|
| Recombinant Bacillus subtilis Uncharacterized deacetylase yojG (yojG) | CSB-YP521781BRJ CSB-EP521781BRJ CSB-BP521781BRJ CSB-MP521781BRJ CSB-EP521781BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis Uncharacterized protein yozD (yozD) | CSB-YP521782BRJ CSB-EP521782BRJ CSB-BP521782BRJ CSB-MP521782BRJ CSB-EP521782BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized HTH-type transcriptional regulator yotL (yotL) | CSB-YP521783BRJ CSB-EP521783BRJ CSB-BP521783BRJ CSB-MP521783BRJ CSB-EP521783BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yotE (yotE) | CSB-YP521784BRJ CSB-EP521784BRJ CSB-BP521784BRJ CSB-MP521784BRJ CSB-EP521784BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yosK (yosK) | CSB-YP521785BRJ CSB-EP521785BRJ CSB-BP521785BRJ CSB-MP521785BRJ CSB-EP521785BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yosG (yosG) | CSB-YP521786BRJ CSB-EP521786BRJ CSB-BP521786BRJ CSB-MP521786BRJ CSB-EP521786BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yosC (yosC) | CSB-YP521787BRJ CSB-EP521787BRJ CSB-BP521787BRJ CSB-MP521787BRJ CSB-EP521787BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yorX (yorX) | CSB-YP521788BRJ CSB-EP521788BRJ CSB-BP521788BRJ CSB-MP521788BRJ CSB-EP521788BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yorQ (yorQ) | CSB-YP521789BRJ CSB-EP521789BRJ CSB-BP521789BRJ CSB-MP521789BRJ CSB-EP521789BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis Probable DNA polymerase yorL (yorL), partial | CSB-YP521790BRJ CSB-EP521790BRJ CSB-BP521790BRJ CSB-MP521790BRJ CSB-EP521790BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yorF (yorF) | CSB-YP521791BRJ CSB-EP521791BRJ CSB-BP521791BRJ CSB-MP521791BRJ CSB-EP521791BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived UPF0714 protein yoqZ (yoqZ) | CSB-YP521792BRJ CSB-EP521792BRJ CSB-BP521792BRJ CSB-MP521792BRJ CSB-EP521792BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yoqU (yoqU) | CSB-YP521793BRJ CSB-EP521793BRJ CSB-BP521793BRJ CSB-MP521793BRJ CSB-EP521793BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yoqN (yoqN) | CSB-YP521794BRJ CSB-EP521794BRJ CSB-BP521794BRJ CSB-MP521794BRJ CSB-EP521794BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yopI (yopI), partial | CSB-YP521795BRJ1 CSB-EP521795BRJ1 CSB-BP521795BRJ1 CSB-MP521795BRJ1 CSB-EP521795BRJ1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived UPF0715 membrane protein yopD (yopD), partial | CSB-YP521796BRJ1 CSB-EP521796BRJ1 CSB-BP521796BRJ1 CSB-MP521796BRJ1 CSB-EP521796BRJ1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yonX (yonX) | CSB-YP521797BRJ CSB-EP521797BRJ CSB-BP521797BRJ CSB-MP521797BRJ CSB-EP521797BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized HTH-type transcriptional regulator yonR (yonR) | CSB-YP521798BRJ CSB-EP521798BRJ CSB-BP521798BRJ CSB-MP521798BRJ CSB-EP521798BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yonI (yonI) | CSB-YP521799BRJ CSB-EP521799BRJ CSB-BP521799BRJ CSB-MP521799BRJ CSB-EP521799BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yonC (yonC) | CSB-YP521800BRJ CSB-EP521800BRJ CSB-BP521800BRJ CSB-MP521800BRJ CSB-EP521800BRJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達載體?
表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
