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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella melitensis biotype 2 Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP505892BWT
CSB-EP505892BWT
CSB-BP505892BWT
CSB-MP505892BWT
CSB-EP505892BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Threonine--tRNA ligase (thrS), partial CSB-YP505893BWT
CSB-EP505893BWT
CSB-BP505893BWT
CSB-MP505893BWT
CSB-EP505893BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP505894BWT
CSB-EP505894BWT
CSB-BP505894BWT
CSB-MP505894BWT
CSB-EP505894BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Anthranilate phosphoribosyltransferase (trpD) CSB-YP505895BWT
CSB-EP505895BWT
CSB-BP505895BWT
CSB-MP505895BWT
CSB-EP505895BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP505896BWT
CSB-EP505896BWT
CSB-BP505896BWT
CSB-MP505896BWT
CSB-EP505896BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP505897BWT
CSB-EP505897BWT
CSB-BP505897BWT
CSB-MP505897BWT
CSB-EP505897BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Adenylate kinase (adk) CSB-YP505898BWT
CSB-EP505898BWT
CSB-BP505898BWT
CSB-MP505898BWT
CSB-EP505898BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L16 (rplP) CSB-YP505899BWT
CSB-EP505899BWT
CSB-BP505899BWT
CSB-MP505899BWT
CSB-EP505899BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S10 (rpsJ) CSB-YP505900BWT
CSB-EP505900BWT
CSB-BP505900BWT
CSB-MP505900BWT
CSB-EP505900BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP505901BWT
CSB-EP505901BWT
CSB-BP505901BWT
CSB-MP505901BWT
CSB-EP505901BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 ATP phosphoribosyltransferase (hisG) CSB-YP505902BWT
CSB-EP505902BWT
CSB-BP505902BWT
CSB-MP505902BWT
CSB-EP505902BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP505903BWT
CSB-EP505903BWT
CSB-BP505903BWT
CSB-MP505903BWT
CSB-EP505903BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Exodeoxyribonuclease 7 large subunit (xseA), partial CSB-YP505904BWT
CSB-EP505904BWT
CSB-BP505904BWT
CSB-MP505904BWT
CSB-EP505904BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP505905BWT
CSB-EP505905BWT
CSB-BP505905BWT
CSB-MP505905BWT
CSB-EP505905BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Ribonuclease P protein component (rnpA) CSB-YP505906BWT
CSB-EP505906BWT
CSB-BP505906BWT
CSB-MP505906BWT
CSB-EP505906BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Undecaprenyl-diphosphatase (uppP), partial CSB-YP505907BWT
CSB-EP505907BWT
CSB-BP505907BWT
CSB-MP505907BWT
CSB-EP505907BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Probable flagellum biosynthesis repressor protein flbT (flbT) CSB-YP505908BWT
CSB-EP505908BWT
CSB-BP505908BWT
CSB-MP505908BWT
CSB-EP505908BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Probable carboxypeptidase PABG_01461 (PABG_01461) CSB-YP505909ERP
CSB-EP505909ERP
CSB-BP505909ERP
CSB-MP505909ERP
CSB-EP505909ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Adenylosuccinate synthetase (PABG_02407) CSB-YP505910ERP
CSB-EP505910ERP
CSB-BP505910ERP
CSB-MP505910ERP
CSB-EP505910ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Vacuolar protein sorting/targeting protein 10 (VPS10), partial CSB-YP505911ERP
CSB-EP505911ERP
CSB-BP505911ERP
CSB-MP505911ERP
CSB-EP505911ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.51tesewang.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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