在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Saccharomyces cerevisiae ERAD-associated E3 ubiquitin-protein ligase HRD1 (HRD1)

  • 中文名稱:
    釀酒酵母HRD1重組蛋白
  • 貨號:
    CSB-CF600065SVG
  • 規格:
  • 來源:
    in vitro E.coli expression system
  • 其他:

產品詳情

  • 基因名:
    HRD1
  • Uniprot No.:
  • 別名:
    HRD1; DER3; YOL013C; ERAD-associated E3 ubiquitin-protein ligase HRD1; HMG-CoA reductase degradation protein 1; RING-type E3 ubiquitin transferase HRD1
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-551
  • 氨基酸序列
    MVPENRRKQLAIFVVVTYLLTFYCVYSATKTSVSFLQVTLKLNEGFNLMVLSIFILLNST LLWQLLTKLLFGELRLIEHEHIFERLPFTIINTLFMSSLFHERYFFTVAFFGLLLLYLKV FHWILKDRLEALLQSINDSTTMKTLIFSRFSFNLVLLAVVDYQIITRCISSIYTNQKSDI ESTSLYLIQVMEFTMLLIDLLNLFLQTCLNFWEFYRSQQSLSNENNHIVHGDPTDENTVE SDQSQPVLNDDDDDDDDDRQFTGLEGKFMYEKAIDVFTRFLKTALHLSMLIPFRMPMMLL KDVVWDILALYQSGTSLWKIWRNNKQLDDTLVTVTVEQLQNSANDDNICIICMDELIHSP NQQTWKNKNKKPKRLPCGHILHLSCLKNWMERSQTCPICRLPVFDEKGNVVQTTFTSNSD ITTQTTVTDSTGIATDQQGFANEVDLLPTRTTSPDIRIVPTQNIDTLAMRTRSTSTPSPT WYTFPLHKTGDNSVGSSRSAYEFLITNSDEKENGIPVKLTIENHEVNSLHGDGGEQIAKK IVIPDKFIQHI
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC1 and UBC7 E2 ligases, and transfers it to substrates promoting their degradation. Mediates the degradation of endoplasmic reticulum proteins (ERQC), also called ER-associated degradation (ERAD). Component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). In ERAD-L, facilitates retrotranslocation of misfolded proteins from the ER lumen through the ER membrane in conjunction with DER1. Both proteins have lateral gates facing each other which form a channel through the ER membrane and which distort the membrane region between the lateral gates, making it much thinner than a normal phospholipid bilayer. Substrates insert into the membrane as a hairpin loop with one strand interacting with DER1 and the other with HRD1. ERAD-L substrates are ubiquitinated through HRD1 in conjunction with the E2 ubiquitin-conjugating enzymes UBC1 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the CDC48-NPL4-UFD1 ATPase complex and targeted to the proteasome. ERAD-M substrates are processed by the same HRD1-HRD3 core complex, but only a subset of the other components is required for ERAD-M.
  • 基因功能參考文獻:
    1. Hrd1 is required for ER to cytosol retrotranslocation of ricin A chain, independently of its ubiquitin ligase activity PMID: 20519439
    2. Hrd1p dislocates ricin A chain to the cytosol from the ER lumen, in a manner independent of its E3 ubiquitin ligase activity. PMID: 20519439
    3. Authors reconstituted retrotranslocation with purified S. cerevisiae proteins, using proteoliposomes containing the multi-spanning ubiquitin ligase Hrd1. Retrotranslocation of the luminal domain of a membrane-spanning substrate is triggered by autoubiquitination of Hrd1. PMID: 27321670
    4. Cytosolic entry of Shiga-like toxin a chain from the yeast endoplasmic reticulum requires catalytically active Hrd1p. PMID: 22829918
    5. Hrd1 therefore likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the translocon. PMID: 22689655
    6. Study shows that Hrd1p is the central membrane component in ERAD-L; its overexpression bypasses the need for the other components of the Hrd1p complex. PMID: 21074049
    7. Data suggest that the Saccharomyces cerevisiae Hrd1 complex manages three distinct substrate classes. PMID: 20212318
    8. Usa1p is required for optimal function and regulation of the Hrd1p endoplasmic reticulum-associated degradation ubiquitin ligase. PMID: 19940128
    9. analysis of RING-E2 fidelity for Hrd1p, a membrane-anchored ubiquitin ligase PMID: 17035235
    10. Systematic analysis of model substrates revealed that glycans mark structural determinants that are sensitive to the overall folding state of the molecule. PMID: 19458187

    顯示更多

    收起更多

  • 亞細胞定位:
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族:
    HRD1 family
  • 數據庫鏈接:

    KEGG: sce:YOL013C

    STRING: 4932.YOL013C



主站蜘蛛池模板: 又湿又紧又大又爽又a视频| 亚洲中文字幕无码一区二区三区| 久久精品国产精品青草| 久久国产热精品波多野结衣av| 久久精品日日躁夜夜躁| 国产又爽又粗又猛的视频| 国产精品ⅴ无码大片在线看 | 国产精品成人无码久久久| 秋霞最新高清无码鲁丝片| 欧美视频网站www色| 偷妻之寂寞难耐2中文字幕 | 人妻少妇69式99偷拍| 性色a∨精品高清在线观看| 乱中年女人伦av二区| 亚洲性无码av在线dvd| 无码中文字幕日韩专区视频| 人妻精品久久久久中文字幕| 欧洲精品无码一区二区三区在线播放 | 国产成人精品视觉盛宴| 国产乱码精品一区二区三区四川人| 欧美性xxxxx极品娇小| 欧美 亚洲 国产 日韩 综aⅴ | 国产成人无码a区在线| 精品无码成人久久久久久| 国产热の有码热の无码视频| 国产精品亚洲专区无码web| 精品国产乱码久久久久久下载| 国产精品18禁污污网站| 亚洲日韩乱码一区二区三区四区| 亚洲精品国产精品制服丝袜 | 国产综合18久久久久久| 久久婷婷国产综合精品| 欧美成人精品a∨在线观看| 欧美亚洲国产第一精品久久| 欧美老熟妇乱xxxxx| 国产 在线 | 日韩| 中国内射xxxx6981少妇| 久久久一本精品99久久精品88| 成人欧美一区二区三区1314| 人人爽人人爽人人片av免费| 亚洲欧美成人精品香蕉网|